5uct

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==Mycobacterium tuberculosis toxin MazF-mt6==
==Mycobacterium tuberculosis toxin MazF-mt6==
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<StructureSection load='5uct' size='340' side='right' caption='[[5uct]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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<StructureSection load='5uct' size='340' side='right'caption='[[5uct]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5uct]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5uct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UCT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uct OCA], [http://pdbe.org/5uct PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uct RCSB], [http://www.ebi.ac.uk/pdbsum/5uct PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uct ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uct OCA], [https://pdbe.org/5uct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uct RCSB], [https://www.ebi.ac.uk/pdbsum/5uct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uct ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MAZF3_MYCTU MAZF3_MYCTU]] Toxic component of a type II toxin-antitoxin (TA) module. Acts as an mRNA and 23S rRNA interferase, cleaving predominantly after the first 2 Us in the sequence 5'-UUCCU-3'; in 23S rRNA only cleaves once in the ribosomal A site in dissociated but not intact ribosomes. Cleavage of 23S rRNA inhibits protein translation; the 23S rRNA region cleaved is involved in tRNA-binding in the A site, 30S and 50S subunit interaction and ribosome recycling factor association (PubMed:23650345). Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. It dramatically increases persister cell formation in M.smegmatis upon challenge with gentamicin or kanamycin. Overexpression leads to bacteriostasis rather than bacteriocide. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE3.<ref>PMID:16611633</ref> <ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20705052</ref> <ref>PMID:23650345</ref> <ref>PMID:25608501</ref>
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[https://www.uniprot.org/uniprot/MAZF3_MYCTU MAZF3_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. Acts as an mRNA and 23S rRNA interferase, cleaving predominantly after the first 2 Us in the sequence 5'-UUCCU-3'; in 23S rRNA only cleaves once in the ribosomal A site in dissociated but not intact ribosomes. Cleavage of 23S rRNA inhibits protein translation; the 23S rRNA region cleaved is involved in tRNA-binding in the A site, 30S and 50S subunit interaction and ribosome recycling factor association (PubMed:23650345). Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. It dramatically increases persister cell formation in M.smegmatis upon challenge with gentamicin or kanamycin. Overexpression leads to bacteriostasis rather than bacteriocide. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE3.<ref>PMID:16611633</ref> <ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20705052</ref> <ref>PMID:23650345</ref> <ref>PMID:25608501</ref>
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== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
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*[[Endonuclease|Endonuclease]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Dunham, C M]]
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[[Category: Large Structures]]
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[[Category: Hoffer, E H]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Hydrolase]]
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[[Category: Dunham CM]]
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[[Category: Rnase]]
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[[Category: Hoffer EH]]
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[[Category: Toxin]]
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[[Category: Tuberculosis]]
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Current revision

Mycobacterium tuberculosis toxin MazF-mt6

PDB ID 5uct

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