5nz7
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Clostridium thermocellum cellodextrin phosphorylase ligand free form== | |
| + | <StructureSection load='5nz7' size='340' side='right'caption='[[5nz7]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5nz7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NZ7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nz7 OCA], [https://pdbe.org/5nz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nz7 RCSB], [https://www.ebi.ac.uk/pdbsum/5nz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nz7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q93HT8_ACETH Q93HT8_ACETH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The GH94 glycoside hydrolase cellodextrin phosphorylase (CDP, EC 2.4.1.49) produces cellodextrin oligomers from short beta-1-->4-glucans and alpha-D-glucose 1-phosphate. Compared to cellobiose phosphorylase (CBP), which produces cellobiose from glucose and alpha-D-glucose 1-phosphate, CDP is biochemically less well characterised. Herein, we investigate the donor and acceptor substrate specificity of recombinant CDP from Ruminiclostridium thermocellum and we isolate and characterise a glucosamine addition product to the cellobiose acceptor with the non-natural donor alpha-D-glucosamine 1-phosphate. In addition, we report the first X-ray crystal structure of CDP, along with comparison to the available structures from CBPs and other closely related enzymes, which contributes to understanding of the key structural features necessary to discriminate between monosaccharide (CBP) and oligosaccharide (CDP) acceptor substrates. | ||
| - | + | Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis.,O'Neill EC, Pergolizzi G, Stevenson CEM, Lawson DM, Nepogodiev SA, Field RA Carbohydr Res. 2017 Jul 21. pii: S0008-6215(17)30359-2. doi:, 10.1016/j.carres.2017.07.005. PMID:28760417<ref>PMID:28760417</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5nz7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Acetivibrio thermocellus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Field RA]] | ||
| + | [[Category: Lawson DM]] | ||
| + | [[Category: Nepogodiev SA]] | ||
| + | [[Category: O'Neill EC]] | ||
| + | [[Category: Pergolizzi G]] | ||
| + | [[Category: Stevenson CEM]] | ||
Current revision
Clostridium thermocellum cellodextrin phosphorylase ligand free form
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