5xmg
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5xmg is ON HOLD Authors: She, Z., Gao, Z.Q. Description: Crystal structure of PA3488 from Pseudomonas aeruginosa [[Category: Unreleased Structures]...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of PA3488 from Pseudomonas aeruginosa== | |
| + | <StructureSection load='5xmg' size='340' side='right' caption='[[5xmg]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5xmg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XMG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XMG FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BZY57_06770, BZY58_00015 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xmg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xmg OCA], [http://pdbe.org/5xmg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xmg RCSB], [http://www.ebi.ac.uk/pdbsum/5xmg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xmg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Widely spread in Gram-negative bacteria, the type VI secretion system (T6SS) secretes many effector-immunity protein pairs to help the bacteria compete against other prokaryotic rivals, and infect their eukaryotic hosts. Tle5 and Tle5B are two phospholipase effector protein secreted by T6SS of Pseudomonas aeruginosa. They can facilitate the bacterial internalization process into human epithelial cells by interacting with Akt protein of the PI3K-Akt signal pathway. Tli5 and PA5086-5088 are cognate immunity proteins of Tle5 and Tle5B, respectively. They can interact with their cognate effector proteins to suppress their virulence. Here, we report the crystal structure of Tli5 at 2.8A resolution and successfully fit it into the Small angle X-ray scattering (SAXS) model of the complete Tle5-Tli5 complex. We identified two important motifs in Tli5 through sequence and structural analysis. One is a conserved loop-beta-hairpin motif that exists in the Tle5 immunity homologs, the other is a long and sharp alpha-alpha motif that directly interacts with Tle5 according to SAXS data. We also distinguished the structural features of Tle5 and Tle5B family immunity proteins. Together, our work provided insights into a novel inhibition mechanism that may enhance our understanding of phospholipase D family proteins. | ||
| - | + | Structural and SAXS analysis of Tle5-Tli5 complex reveals a novel inhibition mechanism of H2-T6SS in Pseudomonas aeruginosa.,Yang XY, Li ZQ, Gao ZQ, Wang WJ, Geng Z, Xu JH, She Z, Dong YH Protein Sci. 2017 Oct;26(10):2083-2091. doi: 10.1002/pro.3246. Epub 2017 Aug 30. PMID:28758353<ref>PMID:28758353</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Gao, Z | + | <div class="pdbe-citations 5xmg" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gao, Z Q]] | ||
[[Category: She, Z]] | [[Category: She, Z]] | ||
| + | [[Category: Immune system]] | ||
| + | [[Category: Immunity protein]] | ||
Current revision
Crystal structure of PA3488 from Pseudomonas aeruginosa
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