5voi

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==X-ray crystal structure of bacterial RNA polymerase and pyrG promoter complex==
==X-ray crystal structure of bacterial RNA polymerase and pyrG promoter complex==
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<StructureSection load='5voi' size='340' side='right' caption='[[5voi]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<StructureSection load='5voi' size='340' side='right'caption='[[5voi]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5voi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_(strain_hb27_/_atcc_baa-163_/_dsm_7039) Thermus thermophilus (strain hb27 / atcc baa-163 / dsm 7039)] and [http://en.wikipedia.org/wiki/Thermus_thermophilus_(strain_hb8_/_atcc_27634_/_dsm_579) Thermus thermophilus (strain hb8 / atcc 27634 / dsm 579)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VOI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5voi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis], [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VOI FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vo8|5vo8]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5voi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voi OCA], [https://pdbe.org/5voi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5voi RCSB], [https://www.ebi.ac.uk/pdbsum/5voi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5voi ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5voi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5voi OCA], [http://pdbe.org/5voi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5voi RCSB], [http://www.ebi.ac.uk/pdbsum/5voi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5voi ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RPOZ_THET8 RPOZ_THET8]] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity). [[http://www.uniprot.org/uniprot/RPOA_THET2 RPOA_THET2]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOC_THET8 RPOC_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOB_THET8 RPOB_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/Q5SKW1_THET8 Q5SKW1_THET8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity).[RuleBase:RU000715] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth (By similarity).[HAMAP-Rule:MF_00963]
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[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Reiterative transcription is a noncanonical form of RNA synthesis in which a nucleotide specified by a single base in the DNA template is repetitively added to the nascent transcript. Here we determined the crystal structure of an RNA polymerase, the bacterial enzyme from Thermus thermophilus, engaged in reiterative transcription during transcription initiation at a promoter resembling the pyrG promoter of Bacillus subtilis The structure reveals that the reiterative transcript detours from the dedicated RNA exit channel and extends toward the main channel of the enzyme, thereby allowing RNA extension without displacement of the promoter recognition sigma-factor. Nascent transcripts containing reiteratively added G residues are eventually extended by nonreiterative transcription, revealing an atypical pathway for the formation of a transcription elongation complex.
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X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.,Murakami KS, Shin Y, Turnbough CL Jr, Molodtsov V Proc Natl Acad Sci U S A. 2017 Aug 1;114(31):8211-8216. doi:, 10.1073/pnas.1702741114. Epub 2017 Jun 26. PMID:28652344<ref>PMID:28652344</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5voi" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Bacillus subtilis]]
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[[Category: Jr, C L.Turnbough]]
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[[Category: Large Structures]]
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[[Category: Molodtsov, V]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Murakami, K S]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Shin, Y]]
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[[Category: Molodtsov V]]
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[[Category: Holoenzyme]]
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[[Category: Murakami KS]]
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[[Category: Reiterative transcription]]
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[[Category: Shin Y]]
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[[Category: Rna polymerase]]
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[[Category: Turnbough Jr CL]]
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[[Category: Thermus thermophilus]]
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[[Category: Transcription-dna-rna complex]]
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Current revision

X-ray crystal structure of bacterial RNA polymerase and pyrG promoter complex

PDB ID 5voi

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