Zika virus subgenomic flaviviral RNA

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<StructureSection load='5tpy' size='350' side='right' caption='[[5tpy]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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<StructureSection load='5tpy' size='350' side='right' caption='exonuclease resistant RNA from Zika virus (PDB id code [[5tpy]])' scene='76/761677/Colored_5tpy/1'>
[[Image:5tpy cartoon.png|left|200px]]
[[Image:5tpy cartoon.png|left|200px]]
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==Crystal structure of an exonuclease resistant RNA from Zika virus==
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{{ABSTRACT_PUBMED_27934765}}
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===Crystal structure of an exonuclease resistant RNA from Zika virus===
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The outbreak of Zika virus (ZIKV) and associated fetal microcephaly mandates efforts to understand the molecular processes of infection. Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to pathogenicity in fetal mice. These viruses make sfRNAs by co-opting a cellular exonuclease via structured RNAs called xrRNAs. We found that ZIKV-infected monkey and human epithelial cells, mouse neurons, and mosquito cells produce sfRNAs. The RNA structure that is responsible for ZIKV sfRNA production forms a complex fold that is likely found in many pathogenic flaviviruses. Mutations that disrupt the structure affect exonuclease resistance in vitro and sfRNA formation during infection. The complete ZIKV xrRNA structure clarifies the mechanism of exonuclease resistance and identifies features that may modulate function in diverse flaviviruses.
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Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease.,Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS Science. 2016 Dec 2;354(6316):1148-1152. Epub 2016 Nov 10. PMID:27934765<ref>PMID:27934765</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5tpy" style="background-color:#fffaf0;"></div>
==About this Structure==
==About this Structure==
===The Basics===
===The Basics===
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Click <scene name='76/761677/Init_5tpy/1'>here</scene> to represent the molecule by the [[DRuMS|DRuMS Color Scheme]].
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It shows the molecule is composed of {{Template:ColorKey Composition RNA}}.
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<!-- Doesn't WORK BECAUSE DOESN'T SEEM TO GET DSSR INFO Click <scene name='76/761677/Showing_bases/2'>here</scene> to see this simplified view where the inter-helical interactions are illustrated an the individual nucleotide bases are indicated by letters.--->
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<scene name='76/761677/Colored_5tpy/1'>Here</scene> the RNA is colored to match the secondary structure in the paper.
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TO DO: NEED AN IMAGE OF SECONDARY STRUCTURE DIAGRAM TO MATCH SCENE.
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A <scene name='76/761677/Alt_colored/1'>ribbon representation of the RNA</scene>.
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In the crystal structure there is additionally observed <scene name='76/761677/With_hexanediol/3'>1,5-hexanediol</scene> and a<scene name='76/761677/W_mg/4'> magnesium ion</scene> bound.
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5' interactions of the RNA highlighted in <scene name='76/761677/5prime_highlight/1'>this scene</scene>.
== PDB Entry ==
== PDB Entry ==
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==Related Structures and Topics==
==Related Structures and Topics==
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*[[Category: Zika virus]]
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*[[Zika virus]]
*[[Viral Infections]]
*[[Viral Infections]]
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==Reference==
==Reference==
<ref group="xtra">PMID:27934765</ref><references group="xtra"/>
<ref group="xtra">PMID:27934765</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
</StructureSection>
[[Category: Zika virus]]
[[Category: Zika virus]]

Current revision

exonuclease resistant RNA from Zika virus (PDB id code 5tpy)

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Page begun with original page seeded for PDB entry 5tpy by OCA on 04:47, January 4, 2017

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Wayne Decatur

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