1vm1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (18:15, 7 May 2025) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1vm1.gif|left|200px]]
 
-
{{Structure
+
==STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM==
-
|PDB= 1vm1 |SIZE=350|CAPTION= <scene name='initialview01'>1vm1</scene>, resolution 2.02&Aring;
+
<StructureSection load='1vm1' size='340' side='right'caption='[[1vm1]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=AKR:ACRYLIC+ACID'>AKR</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=TAZ:TAZOBACTAM'>TAZ</scene>, <scene name='pdbligand=TBE:TAZOBACTAM+INTERMEDIATE'>TBE</scene>
+
<table><tr><td colspan='2'>[[1vm1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VM1 FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
-
|GENE= BLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKR:ACRYLIC+ACID'>AKR</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=TAZ:TAZOBACTAM'>TAZ</scene>, <scene name='pdbligand=TBE:TAZOBACTAM+INTERMEDIATE'>TBE</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vm1 OCA], [https://pdbe.org/1vm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vm1 RCSB], [https://www.ebi.ac.uk/pdbsum/1vm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vm1 ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1shv|1SHV]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vm1 OCA], [http://www.ebi.ac.uk/pdbsum/1vm1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vm1 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/BLA1_KLEPN BLA1_KLEPN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vm/1vm1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vm1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.
-
'''STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM'''
+
Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam.,Kuzin AP, Nukaga M, Nukaga Y, Hujer A, Bonomo RA, Knox JR Biochemistry. 2001 Feb 13;40(6):1861-6. PMID:11327849<ref>PMID:11327849</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1vm1" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.
+
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1VM1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. This structure supersedes the now removed PDB entry 1G56. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VM1 OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam., Kuzin AP, Nukaga M, Nukaga Y, Hujer A, Bonomo RA, Knox JR, Biochemistry. 2001 Feb 13;40(6):1861-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11327849 11327849]
+
-
[[Category: Beta-lactamase]]
+
[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bonomo, R A.]]
+
[[Category: Bonomo RA]]
-
[[Category: Hujer, A.]]
+
[[Category: Hujer A]]
-
[[Category: Knox, J R.]]
+
[[Category: Knox JR]]
-
[[Category: Kuzin, A P.]]
+
[[Category: Kuzin AP]]
-
[[Category: Nukaga, M.]]
+
[[Category: Nukaga M]]
-
[[Category: Nukaga, Y.]]
+
[[Category: Nukaga Y]]
-
[[Category: beta-lactam hydrolase]]
+
-
[[Category: beta-lactamase]]
+
-
[[Category: detergent binding]]
+
-
[[Category: inhibitor design]]
+
-
[[Category: penicillinase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:26:15 2008''
+

Current revision

STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM

PDB ID 1vm1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vm1"
Personal tools