MEP cytidylyltransferase

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<StructureSection load='1i52' size='450' side='right' caption='Structure of E. coli MEP cytidylyltransferase complex with CTP, Mg+2 (small light green) and Ca+2 (large green) (PDB code [[1i52]]). ' scene='59/595219/Cv/1'>
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<StructureSection load='1i52' size='350' side='right' caption='Structure of E. coli MEP cytidylyltransferase complex with CTP, Mg+2 (small light green) and Ca+2 (large green) (PDB code [[1i52]]). ' scene='59/595219/Cv/1'>
== Function ==
== Function ==
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'''MEP cytidylyltransferase''' (MEPCT) catalyzes the conversion of 2C-methyl-D-erythritol 4-phosphate and CTP to 4-(cytidine 5’-diphospho)- 2C-methyl-D-erythritol (CDP-ME) and diphosphate. MEPCT participates in isoprenoid biosynthesis in many bacteria while eukaryotes use the mevalonic acid pathway for the isoprenoed biosynthesis<ref>PMID:11696548</ref>.
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'''MEP cytidylyltransferase''' (MEPCT) catalyzes the conversion of 2C-methyl-D-erythritol 4-phosphate and CTP to 4-(cytidine 5’-diphospho)- 2C-methyl-D-erythritol (CDP-ME) and diphosphate. MEPCT participates in isoprenoid biosynthesis in many bacteria while eukaryotes use the mevalonic acid pathway for the isoprenoed biosynthesis<ref>PMID:11696548</ref>. '''IspD/IspDF''' is a bifunctional enzyme which catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erithritol 4-phophate and the conversion or CDP-ME2P to ME-CPP<ref>PMID:15233799</ref>.
== Relevance ==
== Relevance ==
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== Structural highlights ==
== Structural highlights ==
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The biological assembly of ''E. coli'' MEPCT is <scene name='59/595219/Cv/7'>homodimer</scene>. The <scene name='59/595219/Cv/5'>active site of MEPCT contains an Mg+2 ion</scene><ref>PMID:11427897</ref>. Water molecules shown as red spheres. <scene name='59/595219/Cv/6'>Whole active site</scene>.
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The biological assembly of ''E. coli'' MEPCT is <scene name='59/595219/Cv/8'>homodimer</scene>. The <scene name='59/595219/Cv/9'>active site of MEPCT contains an Mg+2 ion</scene><ref>PMID:11427897</ref>. Water molecules shown as red spheres. <scene name='59/595219/Cv/10'>Whole active site</scene>.
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</StructureSection>
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==3D structures of MEP cytidylyltransferase==
==3D structures of MEP cytidylyltransferase==
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[[MEP cytidylyltransferase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*'''MEP cytidylyltransferase'''
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**[[1inj]], [[1vgt]], [[3n9w]], [[1vgu]] – EcMEPCT – ''Escherichia coli''<br />
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**[[1h3m]] – EcMEPCT (mutant) <br />
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**[[1vgw]], [[1vgz]] – MEPCT – ''Neisseria gonorrhoeae'' <br />
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**[[2px7]] – MEPCT - ''Thermus thermophilus'' <br />
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**[[2vsh]] – SpMEPCT - ''Streptococcus pneumoniae''<br />
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**[[4kt7]] – MEPCT –'' Anaerococcus prevotii''<br />
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**[[4myb]] – MEPCT – ''Francisella tularensis''<br />
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**[[4nai]] – AtMEPCT CT domain – ''Arabidopsis thaliana''<br />
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**[[5mrn]], [[5mrq]] – AtMEPCT CT domain (mutant) <br />
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**[[4ys8]] – BtMEPCT – ''Burkholderia thailandensis'' <br />
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**[[5ddt]], [[5ddv]] – BsMEPCT – ''Bacillus subtilis'' <br />
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*MEP cytidylyltransferase complex with cytidine
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**[[1i52]] – EcMEPCT + CTP <br />
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**[[1ini]] – EcMEPCT + CDP-ME <br />
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**[[1vpa]] – MEPCT + CTP – ''Thermotoga maritima''<br />
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**[[1w77]] – AtMEPCT CT domain (mutant) + CMP <br />
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**[[2xwl]] – MsMEPCT + CTP – ''Mycobacterium smegmatis''<br />
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**[[2xwm]] – MsMEPCT + CMP <br />
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**[[2xwn]], [[3q7u]] – MtMEPCT + CTP - ''Mycobacterium tuberculosis'' <br />
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**[[3q80]] – MtMEPCT + CDP-ME <br />
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**[[4zdq]] – BtMEPCT + CTP <br />
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**[[5hs2]] – BsMEPCT + CTP <br />
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*MEP cytidylyltransferase other complexes
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**[[2wc3]], [[2wc5]], [[2ycm]], [[2yc3]], [[2yc5]] – AtMEPCT CT domain + herbicide<br />
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**[[3okr]] – MtMEPCT + peptide <br />
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**[[4nak]], [[4nal]], [[4nan]] – AtMEPCT CT domain (mutant) + pseudilin derivative<br />
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**[[5mro]], [[5mrp]] – AtMEPCT CT domain (mutant) + pyrimidine derivative<br />
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**[[5mrm]] – AtMEPCT CT domain (mutant) + isoxazole<br />
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**[[2vsi]] – SpMEPCT + pyrimidine derivative <br />
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}}
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== References ==
== References ==

Current revision

Structure of E. coli MEP cytidylyltransferase complex with CTP, Mg+2 (small light green) and Ca+2 (large green) (PDB code 1i52).

Drag the structure with the mouse to rotate

References

  1. ↑ Friedrich R, Fuentes-Prior P, Ong E, Coombs G, Hunter M, Oehler R, Pierson D, Gonzalez R, Huber R, Bode W, Madison EL. Catalytic domain structures of MT-SP1/matriptase, a matrix-degrading transmembrane serine proteinase. J Biol Chem. 2002 Jan 18;277(3):2160-8. Epub 2001 Nov 5. PMID:11696548 doi:10.1074/jbc.M109830200
  2. ↑ Gabrielsen M, Rohdich F, Eisenreich W, Grawert T, Hecht S, Bacher A, Hunter WN. Biosynthesis of isoprenoids: a bifunctional IspDF enzyme from Campylobacter jejuni. Eur J Biochem. 2004 Jul;271(14):3028-35. doi: 10.1111/j.1432-1033.2004.04234.x. PMID:15233799 doi:http://dx.doi.org/10.1111/j.1432-1033.2004.04234.x
  3. ↑ Tsang A, Seidle H, Jawaid S, Zhou W, Smith C, Couch RD. Francisella tularensis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase: kinetic characterization and phosphoregulation. PLoS One. 2011;6(6):e20884. doi: 10.1371/journal.pone.0020884. Epub 2011 Jun 9. PMID:21694781 doi:http://dx.doi.org/10.1371/journal.pone.0020884
  4. ↑ Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP. Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis. Nat Struct Biol. 2001 Jul;8(7):641-8. PMID:11427897 doi:10.1038/89691

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