5v6b

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of GIPC1

Structural highlights

5v6b is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GIPC1_MOUSE Inhibits endothelial cell migration (in vitro). May be involved in G protein-linked signaling (By similarity).

Publication Abstract from PubMed

The GIPC family adaptor proteins mediate endocytosis by tethering cargo proteins to the myosin VI motor. The structural mechanisms for the GIPC/cargo and GIPC/myosin VI interactions remained unclear. PlexinD1, a transmembrane receptor that regulates neuronal and cardiovascular development, is a cargo of GIPCs. GIPC-mediated endocytic trafficking regulates PlexinD1 signaling. Here, we unravel the mechanisms of the interactions among PlexinD1, GIPCs and myosin VI by a series of crystal structures of these proteins in apo or bound states. GIPC1 forms a domain-swapped dimer in an autoinhibited conformation that hinders binding of both PlexinD1 and myosin VI. PlexinD1 binding to GIPC1 releases the autoinhibition, promoting its interaction with myosin VI. GIPCs and myosin VI interact through two distinct interfaces and form an open-ended alternating array. Our data support that this alternating array underlies the oligomerization of the GIPC/Myosin VI complexes in solution and cells.

Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex.,Shang G, Brautigam CA, Chen R, Lu D, Torres-Vazquez J, Zhang X Elife. 2017 May 24;6. pii: e27322. doi: 10.7554/eLife.27322. PMID:28537552^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shang G, Brautigam CA, Chen R, Lu D, Torres-Vazquez J, Zhang X. Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex. Elife. 2017 May 24;6. pii: e27322. doi: 10.7554/eLife.27322. PMID:28537552 doi:http://dx.doi.org/10.7554/eLife.27322

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5v6b"

Categories: Large Structures | Mus musculus | Shang G | Zhang X

@@ Line 1: / Line 1: @@
 ==Crystal structure of GIPC1==
-<StructureSection load='5v6b' size='340' side='right' caption='[[5v6b]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='5v6b' size='340' side='right'caption='[[5v6b]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5v6b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V6B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5v6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V6B FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5v6e|5v6e]], [[5v6h|5v6h]], [[5v6t|5v6t]], [[5v6r|5v6r]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v6b OCA], [http://pdbe.org/5v6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v6b RCSB], [http://www.ebi.ac.uk/pdbsum/5v6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v6b ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v6b OCA], [https://pdbe.org/5v6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v6b RCSB], [https://www.ebi.ac.uk/pdbsum/5v6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v6b ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GIPC1_MOUSE GIPC1_MOUSE]] Inhibits endothelial cell migration (in vitro). May be involved in G protein-linked signaling (By similarity).
+[https://www.uniprot.org/uniprot/GIPC1_MOUSE GIPC1_MOUSE] Inhibits endothelial cell migration (in vitro). May be involved in G protein-linked signaling (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Shang, G]]
+[[Category: Large Structures]]
-[[Category: Zhang, X]]
+[[Category: Mus musculus]]
-[[Category: Binding partener]]
+[[Category: Shang G]]
-[[Category: Protein binding]]
+[[Category: Zhang X]]

5v6b

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Current revision

Crystal structure of GIPC1

Structural highlights

Function

Publication Abstract from PubMed

References

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