5myy

From Proteopedia

(Difference between revisions)

Current revision

Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate

Structural highlights

5myy is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.1Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

Cadmium ions can be effectively used to promote crystal growth and for experimental phasing. Here, the use of cadmium ions as a suitable anomalous scatterer at the standard wavelength of 1 A is demonstrated. The structures of three different proteins were determined using cadmium single-wavelength anomalous dispersion (SAD) phasing. Owing to the strong anomalous signal, the structure of lysozyme could be automatically phased and built using a very low anomalous multiplicity (1.1) and low-completeness (77%) data set. Additionally, it is shown that cadmium ions can easily substitute divalent ions in ATP-divalent cation complexes. This property could be generally applied for phasing experiments of a wide range of nucleotide-binding proteins. Improvements in crystal growth and quality, good anomalous signal at standard wavelengths (i.e. no need to change photon energy) and rapid phasing and refinement using a single data set are benefits that should allow cadmium ions to be widely used for experimental phasing.

Rapid cadmium SAD phasing at the standard wavelength (1 A).,Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A, Meents A Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):581-590. doi:, 10.1107/S2059798317006970. Epub 2017 Jun 30. PMID:28695858^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A, Meents A. Rapid cadmium SAD phasing at the standard wavelength (1 A). Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):581-590. doi:, 10.1107/S2059798317006970. Epub 2017 Jun 30. PMID:28695858 doi:http://dx.doi.org/10.1107/S2059798317006970

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5myy"

Categories: Gallus gallus | Large Structures | Burkhardt A | Meents A | Panneerselvam S

@@ Line 1: / Line 1: @@
 ==Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate==
-<StructureSection load='5myy' size='340' side='right' caption='[[5myy]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
+<StructureSection load='5myy' size='340' side='right'caption='[[5myy]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5myy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MYY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5myy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MYY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5myy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5myy OCA], [http://pdbe.org/5myy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5myy RCSB], [http://www.ebi.ac.uk/pdbsum/5myy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5myy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5myy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5myy OCA], [https://pdbe.org/5myy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5myy RCSB], [https://www.ebi.ac.uk/pdbsum/5myy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5myy ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 5myy" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Lysozyme]]
+[[Category: Gallus gallus]]
-[[Category: Burkhardt, A]]
+[[Category: Large Structures]]
-[[Category: Meents, A]]
+[[Category: Burkhardt A]]
-[[Category: Panneerselvam, S]]
+[[Category: Meents A]]
-[[Category: Cadmium phasing]]
+[[Category: Panneerselvam S]]
-[[Category: Hydrolase]]
-[[Category: Sad phasing]]

5myy

From Proteopedia

Current revision

Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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