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5uxu

From Proteopedia

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YfeA from Yersinia pestis treated with EDTA during purification and crystallization

Structural highlights

5uxu is a 1 chain structure with sequence from Yersinia pestis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.839Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

YFEA_YERPE Part of an ATP-driven transport system YfeABCD for chelated iron.

Publication Abstract from PubMed

Gram-negative bacteria use siderophores, outer membrane receptors, inner membrane transporters and substrate-binding proteins (SBPs) to transport transition metals through the periplasm. The SBPs share a similar protein fold that has undergone significant structural evolution to communicate with a variety of differentially regulated transporters in the cell. In Yersinia pestis, the causative agent of plague, YfeA (YPO2439, y1897), an SBP, is important for full virulence during mammalian infection. To better understand the role of YfeA in infection, crystal structures were determined under several environmental conditions with respect to transition-metal levels. Energy-dispersive X-ray spectroscopy and anomalous X-ray scattering data show that YfeA is polyspecific and can alter its substrate specificity. In minimal-media experiments, YfeA crystals grown after iron supplementation showed a threefold increase in iron fluorescence emission over the iron fluorescence emission from YfeA crystals grown from nutrient-rich conditions, and YfeA crystals grown after manganese supplementation during overexpression showed a fivefold increase in manganese fluorescence emission over the manganese fluorescence emission from YfeA crystals grown from nutrient-rich conditions. In all experiments, the YfeA crystals produced the strongest fluorescence emission from zinc and could not be manipulated otherwise. Additionally, this report documents the discovery of a novel surface metal-binding site that prefers to chelate zinc but can also bind manganese. Flexibility across YfeA crystal forms in three loops and a helix near the buried metal-binding site suggest that a structural rearrangement is required for metal loading and unloading.

Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites.,Radka CD, DeLucas LJ, Wilson LS, Lawrenz MB, Perry RD, Aller SG Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):557-572. doi:, 10.1107/S2059798317006349. Epub 2017 Jun 30. PMID:28695856^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Radka CD, DeLucas LJ, Wilson LS, Lawrenz MB, Perry RD, Aller SG. Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites. Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):557-572. doi:, 10.1107/S2059798317006349. Epub 2017 Jun 30. PMID:28695856 doi:http://dx.doi.org/10.1107/S2059798317006349

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5uxu"

Categories: Large Structures | Yersinia pestis | Aller SG | DeLucas LJ | Radka CD

@@ Line 1: / Line 1: @@
 ==YfeA from Yersinia pestis treated with EDTA during purification and crystallization==
-<StructureSection load='5uxu' size='340' side='right' caption='[[5uxu]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
+<StructureSection load='5uxu' size='340' side='right'caption='[[5uxu]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5uxu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5uxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UXU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.839&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uxs|5uxs]], [[5uy0|5uy0]], [[5uy4|5uy4]], [[5uy5|5uy5]], [[5uya|5uya]], [[5uyb|5uyb]], [[5uyd|5uyd]], [[5uye|5uye]], [[5uyf|5uyf]], [[5uyg|5uyg]], [[5uyh|5uyh]], [[5uyc|5uyc]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxu OCA], [http://pdbe.org/5uxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxu RCSB], [http://www.ebi.ac.uk/pdbsum/5uxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxu ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxu OCA], [https://pdbe.org/5uxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uxu RCSB], [https://www.ebi.ac.uk/pdbsum/5uxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE]] Part of an ATP-driven transport system YfeABCD for chelated iron.
+[https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE] Part of an ATP-driven transport system YfeABCD for chelated iron.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Aller, S G]]
+[[Category: Large Structures]]
-[[Category: DeLucas, L J]]
+[[Category: Yersinia pestis]]
-[[Category: Radka, C D]]
+[[Category: Aller SG]]
-[[Category: Metal transport]]
+[[Category: DeLucas LJ]]
-[[Category: Polyspecific]]
+[[Category: Radka CD]]
-[[Category: Transition metal]]
-[[Category: Transport]]

5uxu

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Current revision

YfeA from Yersinia pestis treated with EDTA during purification and crystallization

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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