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5xsy

From Proteopedia

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Structure of the Nav1.4-beta1 complex from electric eel

5xsy, resolution 4.00Å

Structural highlights

5xsy is a 2 chain structure with sequence from Electrophorus electricus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCNA_ELEEL] Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.^[1]

Publication Abstract from PubMed

Voltage-gated sodium (Nav) channels initiate and propagate action potentials. Here, we present the cryo-EM structure of EeNav1.4, the Nav channel from electric eel, in complex with the beta1 subunit at 4.0 A resolution. The immunoglobulin domain of beta1 docks onto the extracellular L5I and L6IV loops of EeNav1.4 via extensive polar interactions, and the single transmembrane helix interacts with the third voltage-sensing domain (VSDIII). The VSDs exhibit "up" conformations, while the intracellular gate of the pore domain is kept open by a digitonin-like molecule. Structural comparison with closed NavPaS shows that the outward transfer of gating charges is coupled to the iris-like pore domain dilation through intricate force transmissions involving multiple channel segments. The IFM fast inactivation motif on the III-IV linker is plugged into the corner enclosed by the outer S4-S5 and inner S6 segments in repeats III and IV, suggesting a potential allosteric blocking mechanism for fast inactivation.

Structure of the Nav1.4-beta1 Complex from Electric Eel.,Yan Z, Zhou Q, Wang L, Wu J, Zhao Y, Huang G, Peng W, Shen H, Lei J, Yan N Cell. 2017 Jul 27;170(3):470-482.e11. doi: 10.1016/j.cell.2017.06.039. Epub 2017 , Jul 20. PMID:28735751^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Noda M, Numa S. Structure and function of sodium channel. J Recept Res. 1987;7(1-4):467-97. PMID:2442385
↑ Yan Z, Zhou Q, Wang L, Wu J, Zhao Y, Huang G, Peng W, Shen H, Lei J, Yan N. Structure of the Nav1.4-beta1 Complex from Electric Eel. Cell. 2017 Jul 27;170(3):470-482.e11. doi: 10.1016/j.cell.2017.06.039. Epub 2017 , Jul 20. PMID:28735751 doi:http://dx.doi.org/10.1016/j.cell.2017.06.039

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xsy"

@@ Line 1: / Line 1: @@
 ==Structure of the Nav1.4-beta1 complex from electric eel==
-<StructureSection load='5xsy' size='340' side='right' caption='[[5xsy]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
+<SX load='5xsy' size='340' side='right' viewer='molstar' caption='[[5xsy]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xsy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Electrophorus_electricus Electrophorus electricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XSY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xsy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Electrophorus_electricus Electrophorus electricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XSY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XSY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xsy OCA], [http://pdbe.org/5xsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xsy RCSB], [http://www.ebi.ac.uk/pdbsum/5xsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xsy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xsy OCA], [http://pdbe.org/5xsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xsy RCSB], [http://www.ebi.ac.uk/pdbsum/5xsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xsy ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 5xsy" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ion channels 3D structures|Ion channels 3D structures]]
 == References ==
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Electrophorus electricus]]
+[[Category: Large Structures]]
 [[Category: Wu, J P]]
 [[Category: Yan, N]]

5xsy

From Proteopedia

Current revision

Structure of the Nav1.4-beta1 complex from electric eel

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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