5w3t

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(New page: ==Crystal structure of PopP2 in complex with IP6== <StructureSection load='5w3t' size='340' side='right' caption='5w3t, resolution 2.15&Aring;' scene=''> == Structural ...)
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==Crystal structure of PopP2 in complex with IP6==
==Crystal structure of PopP2 in complex with IP6==
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<StructureSection load='5w3t' size='340' side='right' caption='[[5w3t]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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<StructureSection load='5w3t' size='340' side='right'caption='[[5w3t]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5w3t]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5w3t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W3T FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w3x|5w3x]], [[5w40|5w40]], [[5w3y|5w3y]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3t OCA], [http://pdbe.org/5w3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3t RCSB], [http://www.ebi.ac.uk/pdbsum/5w3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3t ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3t OCA], [https://pdbe.org/5w3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w3t RCSB], [https://www.ebi.ac.uk/pdbsum/5w3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3t ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0S4VB05_RALSL A0A0S4VB05_RALSL]
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The Yersinia outer protein J (YopJ) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. However, the underlying mechanism is unclear. Here, we report the crystal structures of PopP2, a YopJ effector produced by the plant pathogen Ralstonia solanacearum, in complex with inositol hexaphosphate (InsP6), acetyl-coenzyme A (AcCoA) and/or substrate Resistance to Ralstonia solanacearum 1 (RRS1-R)WRKY. PopP2 recognizes the WRKYGQK motif of RRS1-RWRKY to position a targeted lysine in the active site for acetylation. Importantly, the PopP2-RRS1-RWRKY association is allosterically regulated by InsP6 binding, suggesting a previously unidentified role of the eukaryote-specific cofactor in substrate interaction. Furthermore, we provide evidence for the reaction intermediate of PopP2-mediated acetylation, an acetyl-cysteine covalent adduct, lending direct support to the 'ping-pong'-like catalytic mechanism proposed for YopJ effectors. Our study provides critical mechanistic insights into the virulence activity of YopJ class of acetyltransferases.
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Mechanism of host substrate acetylation by a YopJ family effector.,Zhang ZM, Ma KW, Gao L, Hu Z, Schwizer S, Ma W, Song J Nat Plants. 2017 Jul 24;3:17115. doi: 10.1038/nplants.2017.115. PMID:28737762<ref>PMID:28737762</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5w3t" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Song, J]]
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[[Category: Large Structures]]
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[[Category: Zhang, Z M]]
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[[Category: Ralstonia solanacearum]]
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[[Category: Ip6]]
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[[Category: Song J]]
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[[Category: Popp2]]
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[[Category: Zhang ZM]]
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[[Category: Transcription]]
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[[Category: Yopj effector]]
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Current revision

Crystal structure of PopP2 in complex with IP6

PDB ID 5w3t

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