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5xon

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(New page: ==RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS== <StructureSection load='5xon' size='340' side='right' caption='5xon, resolution 3.83&Aring;' scene=...)
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==RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS==
==RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS==
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<StructureSection load='5xon' size='340' side='right' caption='[[5xon]], [[Resolution|resolution]] 3.83&Aring;' scene=''>
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<SX load='5xon' size='340' side='right' viewer='molstar' caption='[[5xon]], [[Resolution|resolution]] 3.83&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5xon]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Komagataella_phaffii_(strain_gs115_/_atcc_20864) Komagataella phaffii (strain gs115 / atcc 20864)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XON FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5xon]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii_GS115 Komagataella phaffii GS115]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XON FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.83&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xon OCA], [http://pdbe.org/5xon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xon RCSB], [http://www.ebi.ac.uk/pdbsum/5xon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xon ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xon OCA], [https://pdbe.org/5xon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xon RCSB], [https://www.ebi.ac.uk/pdbsum/5xon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xon ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/C4R0E6_KOMPG C4R0E6_KOMPG]] The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.[PIRNR:PIRNR025023] [[http://www.uniprot.org/uniprot/C4QZQ7_KOMPG C4QZQ7_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU363031] [[http://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279] [[http://www.uniprot.org/uniprot/C4QY79_KOMPG C4QY79_KOMPG]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[PIRNR:PIRNR005586]
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[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex (EC) for processive transcription. Here, we report the structure of the Pol II EC bound with the basal elongation factors Spt4/5, Elf1, and TFIIS. Spt4/5 (the Spt4/Spt5 complex) and Elf1 modify a wide area of the Pol II surface. Elf1 bridges the Pol II central cleft, completing the "DNA entry tunnel" for downstream DNA. Spt4 and the Spt5 NGN and KOW1 domains encircle the upstream DNA, constituting a "DNA exit tunnel". The Spt5 KOW4 and KOW5 domains augment the "RNA exit tunnel", directing the exiting nascent RNA. Thus, the EC establishes a completely different transcription/regulation platform from that of the initiation complexes.
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Structure of the complete elongation complex of RNA polymerase II with basal factors.,Ehara H, Yokoyama T, Shigematsu H, Yokoyama S, Shirouzu M, Sekine SI Science. 2017 Aug 3. pii: eaan8552. doi: 10.1126/science.aan8552. PMID:28775211<ref>PMID:28775211</ref>
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==See Also==
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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</div>
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<div class="pdbe-citations 5xon" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Komagataella phaffii GS115]]
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[[Category: Ehara, H]]
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[[Category: Large Structures]]
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[[Category: Sekine, S]]
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[[Category: Ehara H]]
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[[Category: Shigematsu, H]]
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[[Category: Sekine S]]
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[[Category: Shirouzu, M]]
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[[Category: Shigematsu H]]
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[[Category: Yokoyama, T]]
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[[Category: Shirouzu M]]
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[[Category: Complex]]
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[[Category: Yokoyama T]]
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[[Category: Transcription]]
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[[Category: Transcription-rna complex]]
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Current revision

RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS

5xon, resolution 3.83Å

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