5ds5

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==Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg==
==Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg==
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<StructureSection load='5ds5' size='340' side='right' caption='[[5ds5]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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<StructureSection load='5ds5' size='340' side='right'caption='[[5ds5]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ds5]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DS5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ds5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DS5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.951&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p6i|4p6i]], [[5ds6|5ds6]], [[5ds4|5ds4]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ds5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ds5 OCA], [http://pdbe.org/5ds5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ds5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ds5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ds5 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ds5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ds5 OCA], [https://pdbe.org/5ds5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ds5 RCSB], [https://www.ebi.ac.uk/pdbsum/5ds5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ds5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CAS1_ECOLI CAS1_ECOLI]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas1 is thought to be involved in CRISPR adaptation, the first stage of CRISPR immunity. Might be involved in the addition/removal of CRISPR spacers. Endonucleolytically cleaves linear ssRNA, ssDNA and short (34 base) dsDNA as well as branched DNA substrates such as Holliday junctions, replication forks and 5'-flap DNA substrates. Genetic interactions suggest Cas1 interacts with components of the RecBC and RuvB DNA repair systems.<ref>PMID:21255106</ref> [[http://www.uniprot.org/uniprot/CAS2_ECOLI CAS2_ECOLI]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).<ref>PMID:21255106</ref>
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[https://www.uniprot.org/uniprot/CAS1_ECOLI CAS1_ECOLI] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas1 is thought to be involved in CRISPR adaptation, the first stage of CRISPR immunity. Might be involved in the addition/removal of CRISPR spacers. Endonucleolytically cleaves linear ssRNA, ssDNA and short (34 base) dsDNA as well as branched DNA substrates such as Holliday junctions, replication forks and 5'-flap DNA substrates. Genetic interactions suggest Cas1 interacts with components of the RecBC and RuvB DNA repair systems.<ref>PMID:21255106</ref>
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
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*[[Endonuclease|Endonuclease]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Doudna, J A]]
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[[Category: Escherichia coli K-12]]
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[[Category: Engelman, A N]]
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[[Category: Escherichia virus M13]]
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[[Category: Harrington, L B]]
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[[Category: Large Structures]]
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[[Category: Kranzusch, P J]]
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[[Category: Doudna JA]]
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[[Category: Nunez, J K]]
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[[Category: Engelman AN]]
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[[Category: Adaptive immunity]]
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[[Category: Harrington LB]]
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[[Category: Clustered regularly interspaced short palindromic repeat]]
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[[Category: Kranzusch PJ]]
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[[Category: Crispr-associated protein]]
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[[Category: Nunez JK]]
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[[Category: Crispr-cas system]]
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[[Category: Dna binding protein]]
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[[Category: Endodeoxyribonuclease]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Integrase]]
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Current revision

Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg

PDB ID 5ds5

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