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Nitroreductase
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| - | <StructureSection load=' | + | <StructureSection load='' size='350' side='right' caption='E. coli nitroreductase dimer containing FMN and nicotinic acid (PDB entry [[1icr]])' scene='48/489319/Cv/1'> |
== Function == | == Function == | ||
| - | '''Nitroreductase''' (NR) is involved in the reduction of nitrogen-containing compounds including those containing NO<sub>2</sub> group. The NO<sub>2</sub> group is reduced to hydroxylamine<ref>PMID:11491290</ref>. NR uses FMN as cofactor. Among NRs are the oxygen-insensitive NAD(P)H NR and NADH dehydrogenase. | + | '''Nitroreductase''' or '''oxygen-insensitive NAD(P)H nitroreductase''' (NR) is involved in the reduction of nitrogen-containing compounds including those containing NO<sub>2</sub> group. The NO<sub>2</sub> group is reduced to hydroxylamine<ref>PMID:11491290</ref>. NR uses FMN as cofactor. Among NRs are the oxygen-insensitive NAD(P)H NR and NADH dehydrogenase. |
== Relevance == | == Relevance == | ||
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== Structural highlights == | == Structural highlights == | ||
| - | NR <scene name='48/489319/Cv/ | + | NR <scene name='48/489319/Cv/4'>cofactor FMN is bound directly to one subunit</scene> while the <scene name='48/489319/Cv/5'>ligand - nicotinic acid - interacts with both subunits</scene>. Water molecules are shown as red spheres. |
| - | + | ==3D structures of nitroreductase== | |
| + | [[Nitroreductase 3D structures]] | ||
</StructureSection> | </StructureSection> | ||
| - | ==3D structures of nitroreductase== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *Nitroreductase | ||
| - | |||
| - | **[[1f5v ]], [[1oo5]] – EcNR – ''Escherichia coli''<br /> | ||
| - | **[[3x21]], [[3x22]] – EcNR (mutant)<br /> | ||
| - | **[[3bm1]], [[1ds7]] – EcNR minimal<br /> | ||
| - | **[[3bm2]] – EcNR minimal -FMN<br /> | ||
| - | **[[1nec]], [[1kqd]] – EclNR – ''Enterobacter cloacae''<br /> | ||
| - | **[[1ywq]], [[2i7h]] – NR – ''Bacillus cereus''<br /> | ||
| - | **[[2b67]] – NR – ''Streptococcus pneumonia''<br /> | ||
| - | **[[3ek3]], [[3hoi]] – NR – ''Bacterioides fragilis''<br /> | ||
| - | **[[3g14]] – NR – ''Clostridium novyi''<br /> | ||
| - | **[[3h4o]], [[3koq]] - NR – ''Clostridium difficile''<br /> | ||
| - | **[[3gr3]] – NR – ''Bartonella henselae''<br /> | ||
| - | **[[3hzn]] – NR – ''Salmonella typhimurium''<br /> | ||
| - | **[[3k6h]] – NR – ''Agrobacterium tumefaciens''<br /> | ||
| - | **[[2wzv]], [[2ymv]] – MsNR – ''Mycobacterium smegmatis''<br /> | ||
| - | **[[3of4]] – NR FMN/FAD – ''Idiomarina loihiensis''<br /> | ||
| - | **[[3n2s]] – NR – ''Bacillus subtilis''<br /> | ||
| - | **[[3pxv]] – NR – ''Desulfitobacterium hafniense''<br /> | ||
| - | **[[3qdl]] – NR – ''Helicobacter pylori''<br /> | ||
| - | **[[3r5l]], [[3r5p]] - MtNR – ''Mycobacterium tuberculosis''<br /> | ||
| - | **[[3r5r]], [[3r5w]] – MtNR F420<br /> | ||
| - | **[[3ge6]] – NR – ''Exiguobacterium sibiricum''<br /> | ||
| - | **[[4dn2]] – NR – ''Geobacter metallireducens''<br /> | ||
| - | **[[5ko7]], [[5ko8]], [[5krd]] – NR – ''Haliscomenobacter hydrossis''<br /> | ||
| - | |||
| - | *Copper-induced nitroreductase | ||
| - | |||
| - | **[[2wqf]] – LlNR – ''Lactococcus lactis''<br /> | ||
| - | **[[4bn6]] – LlNR + chloramphenicol<br /> | ||
| - | **[[4bn7]], [[4bn8]] – LlNR + phenol derivative<br /> | ||
| - | **[[4bn9]] – LlNR + nicotinic acid<br /> | ||
| - | **[[4bnb]] – LlNR + nitroquinoline<br /> | ||
| - | |||
| - | *Nitroreductase binary complex | ||
| - | **[[1kqb]], [[5j8g]]– EclNR + benzoate derivative<br /> | ||
| - | **[[1kqc]] – EclNR + acetate<br /> | ||
| - | **[[1ylr]], [[1ylu]] - EcNR + acetate<br /> | ||
| - | **[[1icr]], [[1icu]], [[1icv]], [[5j8d]] – EcNR + NAD derivative<br /> | ||
| - | **[[1ooq]] – EcNR + dicoumarol<br /> | ||
| - | **[[1oo6]], [[1oon]], [[1idt]] – EcNR + benzamide derivative<br /> | ||
| - | **[[1yki]] – EcNR + antibiotic<br /> | ||
| - | **[[2wzw]] - MsNR + NADPH | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Lovering AL, Hyde EI, Searle PF, White SA. The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution. J Mol Biol. 2001 May 25;309(1):203-13. PMID:11491290 doi:10.1006/jmbi.2001.4653
- ↑ Williams EM, Little RF, Mowday AM, Rich MH, Chan-Hyams JV, Copp JN, Smaill JB, Patterson AV, Ackerley DF. Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility. Biochem J. 2015 Oct 15;471(2):131-53. doi: 10.1042/BJ20150650. PMID:26431849 doi:http://dx.doi.org/10.1042/BJ20150650
