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| | ==Crystal Structure of eiF4E from C. melo bound to a eIF4G peptide== | | ==Crystal Structure of eiF4E from C. melo bound to a eIF4G peptide== |
| - | <StructureSection load='5me5' size='340' side='right' caption='[[5me5]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='5me5' size='340' side='right'caption='[[5me5]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5me5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ME5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ME5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5me5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucumis_melo Cucumis melo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ME5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ME5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5me5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5me5 OCA], [http://pdbe.org/5me5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5me5 RCSB], [http://www.ebi.ac.uk/pdbsum/5me5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5me5 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5me5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5me5 OCA], [https://pdbe.org/5me5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5me5 RCSB], [https://www.ebi.ac.uk/pdbsum/5me5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5me5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/IF4E1_CUCME IF4E1_CUCME] Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:17026540, PubMed:28522457). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:17026540, PubMed:28522457). Key component of recessive resistance to potyviruses and Tombusviridae genus Carmovirus such as melon necrotic spot virus (MNSV) (PubMed:17026540).<ref>PMID:17026540</ref> <ref>PMID:28522457</ref> (Microbial infection) Susceptibility host factor required for viral infection by recruiting viral RNAs, including uncapped and non-polyadenylated RNA, to the host ribosomal complex via an interaction with viral genome-linked protein (VPg).<ref>PMID:17026540</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5me5" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5me5" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aranda-Regules, M]] | + | [[Category: Cucumis melo]] |
| - | [[Category: Miras, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Querol-Audi, J]] | + | [[Category: Aranda-Regules M]] |
| - | [[Category: Silva, C]] | + | [[Category: Miras M]] |
| - | [[Category: Truniger, V]] | + | [[Category: Querol-Audi J]] |
| - | [[Category: Verdaguer, N]] | + | [[Category: Silva C]] |
| - | [[Category: Translation]]
| + | [[Category: Truniger V]] |
| - | [[Category: Translation initiation eif4f complex]]
| + | [[Category: Verdaguer N]] |
| Structural highlights
Function
IF4E1_CUCME Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:17026540, PubMed:28522457). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:17026540, PubMed:28522457). Key component of recessive resistance to potyviruses and Tombusviridae genus Carmovirus such as melon necrotic spot virus (MNSV) (PubMed:17026540).[1] [2] (Microbial infection) Susceptibility host factor required for viral infection by recruiting viral RNAs, including uncapped and non-polyadenylated RNA, to the host ribosomal complex via an interaction with viral genome-linked protein (VPg).[3]
Publication Abstract from PubMed
The association-dissociation of the cap-binding protein eukaryotic translation initiation factor 4E (eIF4E) with eIF4G is a key control step in eukaryotic translation. The paradigm on the eIF4E-eIF4G interaction states that eIF4G binds to the dorsal surface of eIF4E through a single canonical alpha-helical motif, while metazoan eIF4E-binding proteins (m4E-BPs) advantageously compete against eIF4G via bimodal interactions involving this canonical motif and a second noncanonical motif of the eIF4E surface. Metazoan eIF4Gs share this extended binding interface with m4E-BPs, with significant implications on the understanding of translation regulation and the design of therapeutic molecules. Here we show the high-resolution structure of melon (Cucumis melo) eIF4E in complex with a melon eIF4G peptide and propose the first eIF4E-eIF4G structural model for plants. Our structural data together with functional analyses demonstrate that plant eIF4G binds to eIF4E through both the canonical and noncanonical motifs, similarly to metazoan eIF4E-eIF4G complexes. As in the case of metazoan eIF4E-eIF4G, this may have very important practical implications, as plant eIF4E-eIF4G is also involved in a significant number of plant diseases. In light of our results, a universal eukaryotic bipartite mode of binding to eIF4E is proposed.
Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation.,Miras M, Truniger V, Silva C, Verdaguer N, Aranda MA, Querol-Audi J Plant Physiol. 2017 Jul;174(3):1476-1491. doi: 10.1104/pp.17.00193. Epub 2017 May, 18. PMID:28522457[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nieto C, Morales M, Orjeda G, Clepet C, Monfort A, Sturbois B, Puigdomènech P, Pitrat M, Caboche M, Dogimont C, Garcia-Mas J, Aranda MA, Bendahmane A. An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon. Plant J. 2006 Nov;48(3):452-62. PMID:17026540 doi:10.1111/j.1365-313X.2006.02885.x
- ↑ Miras M, Truniger V, Silva C, Verdaguer N, Aranda MA, Querol-Audi J. Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation. Plant Physiol. 2017 Jul;174(3):1476-1491. doi: 10.1104/pp.17.00193. Epub 2017 May, 18. PMID:28522457 doi:http://dx.doi.org/10.1104/pp.17.00193
- ↑ Nieto C, Morales M, Orjeda G, Clepet C, Monfort A, Sturbois B, Puigdomènech P, Pitrat M, Caboche M, Dogimont C, Garcia-Mas J, Aranda MA, Bendahmane A. An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon. Plant J. 2006 Nov;48(3):452-62. PMID:17026540 doi:10.1111/j.1365-313X.2006.02885.x
- ↑ Miras M, Truniger V, Silva C, Verdaguer N, Aranda MA, Querol-Audi J. Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation. Plant Physiol. 2017 Jul;174(3):1476-1491. doi: 10.1104/pp.17.00193. Epub 2017 May, 18. PMID:28522457 doi:http://dx.doi.org/10.1104/pp.17.00193
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