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1wac

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[[Image:1wac.gif|left|200px]]
 
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{{Structure
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==Back-priming mode of Phi6 RNA-dependent RNA polymerase==
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|PDB= 1wac |SIZE=350|CAPTION= <scene name='initialview01'>1wac</scene>, resolution 3.0&Aring;
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<StructureSection load='1wac' size='340' side='right'caption='[[1wac]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1wac]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [https://pdbe.org/1wac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB], [https://www.ebi.ac.uk/pdbsum/1wac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wac ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [http://www.ebi.ac.uk/pdbsum/1wac PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB]</span>
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[https://www.uniprot.org/uniprot/RDRP_BPPH6 RDRP_BPPH6]
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}}
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''
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==Overview==
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The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
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==About this Structure==
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Back-priming mode of phi6 RNA-dependent RNA polymerase.,Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773<ref>PMID:15659773</ref>
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1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773]
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</div>
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[[Category: Pseudomonas phage phi6]]
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<div class="pdbe-citations 1wac" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Bamford, D H.]]
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[[Category: Grimes, J M.]]
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[[Category: Laurila, M R.L.]]
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[[Category: Salgado, P S.]]
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[[Category: Stuart, D I.]]
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[[Category: phi6 rna-dependent rna polymerase]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:28 2008''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas virus phi6]]
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[[Category: Bamford DH]]
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[[Category: Grimes JM]]
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[[Category: Laurila MRL]]
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[[Category: Salgado PS]]
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[[Category: Stuart DI]]

Current revision

Back-priming mode of Phi6 RNA-dependent RNA polymerase

PDB ID 1wac

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