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Plasminogen activator
From Proteopedia
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| - | <StructureSection load='' size=' | + | <StructureSection load='' size='350' side='right' caption='Human tissue plasminogen activator light chain catalytic domain (sky blue and green), heavy chain catalytic domain fragment (yellow and pink) complex with benzamidine, [[1a5h]]' scene='47/476004/Cv/1' pspeed='8' > |
== Function == | == Function == | ||
'''Plasminogen activator''' (PLA) is a serine protease which converts plasminogen to plasmin. TPA structure contains heavy and light chains (HC, LC). PLA is inhibited by PLA inhibitor 1 and 2. | '''Plasminogen activator''' (PLA) is a serine protease which converts plasminogen to plasmin. TPA structure contains heavy and light chains (HC, LC). PLA is inhibited by PLA inhibitor 1 and 2. | ||
| - | *'''Tissue PLA''' (TPA) which is involved in breakdown of blood clots | + | *'''Tissue PLA''' (TPA) which is involved in breakdown of blood clots. |
| + | *'''Urokinase-type PLA''' see [[Urokinase]].<br /> | ||
*'''Coagulase/fibrinolysin''' is a plasminogen activator from ''Yersinia pestis'' which possesses coagulase activity is responsible for degradation of plasmid-coded outer membrane proteins<ref>PMID:2843471</ref>. | *'''Coagulase/fibrinolysin''' is a plasminogen activator from ''Yersinia pestis'' which possesses coagulase activity is responsible for degradation of plasmid-coded outer membrane proteins<ref>PMID:2843471</ref>. | ||
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Treatment with tissue PLA for acute ischemic stroke is beneficial<ref>PMID:7477192</ref>. | Treatment with tissue PLA for acute ischemic stroke is beneficial<ref>PMID:7477192</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
| - | *<scene name='47/476004/Cv/ | + | *<scene name='47/476004/Cv/4'>Interactions between tissue plasminogen activator light chain catalytic domain and heavy chain catalytic domain fragment</scene>. Water molecules are shown as red spheres. |
| - | *<scene name='47/476004/Cv/ | + | *<scene name='47/476004/Cv/5'>Benzamidine binding site</scene>. |
</StructureSection> | </StructureSection> | ||
==3D structures of plasminogen activator== | ==3D structures of plasminogen activator== | ||
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**[[1bqy]] – PLA – Pit viper | **[[1bqy]] – PLA – Pit viper | ||
| - | *Tissue plasminogen activator | + | *Tissue plasminogen activator; Domains – fibrin-binding 36-85; kringle 1 69-153; kringle 2 209-298; catalytic 311-561 |
**[[1a5h]] – hTPA HC+LC + benzamidine – human<br /> | **[[1a5h]] – hTPA HC+LC + benzamidine – human<br /> | ||
**[[1rtf]] - hTPA HC+LC<br /> | **[[1rtf]] - hTPA HC+LC<br /> | ||
**[[1tpg]] – hTPA F1-G - NMR<br /> | **[[1tpg]] – hTPA F1-G - NMR<br /> | ||
| - | **[[1bda]] – hTPA catalytic domain (mutant) + EGR-CMK<br /> | ||
**[[1pk2]] – hTPA kringle 2 domain + 6-aminohexanoic acid – NMR<br /> | **[[1pk2]] – hTPA kringle 2 domain + 6-aminohexanoic acid – NMR<br /> | ||
**[[1tpk]], [[1pml]] - hTPA kringle 2 domain<br /> | **[[1tpk]], [[1pml]] - hTPA kringle 2 domain<br /> | ||
| - | **[[1kdu]] - hTPA kringle domain – NMR<br /> | + | **[[1kdu]] - hTPA kringle 1 domain – NMR<br /> |
| - | **[[1tpm]], [[1tpn]] - hTPA fibrin-binding domain – NMR | + | **[[1tpm]], [[1tpn]] - hTPA fibrin-binding domain – NMR<br /> |
| + | **[[1bda]] – hTPA catalytic domain (mutant) + EGR-CMK<br /> | ||
| + | **[[5brr]], [[5zlz]] - hTPA catalytic domain (mutant) + plasminogen activator inhibitor 1<br /> | ||
| + | |||
| + | *Urokinase-type plasminogen activator | ||
| + | |||
| + | **[[4xsk]] - hUPA catalytic domain (mutant) <br /> | ||
| + | **[[5xg4]] - hUPA + quercetin<br /> | ||
| + | **[[5lhs]] - mUPA catalytic domain - mouse<br /> | ||
| + | **[[5lhn]], [[5lhp]], [[5lhq]], [[5lhr]], [[5hgg]] - mUPA catalytic domain (mutant) + antibody<br /> | ||
| + | **[[4zks]], [[4zkn]], [[4zko]], [[4zkr]] - hUPA catalytic domain (mutant) + UPAIN-1-W3A<br /> | ||
| + | **[[4zhl]], [[4x1s]], [[4x1r]], [[4x1q]], [[4x1p]], [[4x1n]], [[4x0w]] - hUPA catalytic domain (mutant) + MUPAIN-1-IG<br /> | ||
| + | **[[4zhm]] - hUPA catalytic domain (mutant) + MUPAIN-1-16-IG<br /> | ||
| + | **[[4os7]], [[4os6]], [[4os5]], [[4os4]], [[4os2]], [[4os1]], [[4mny]], [[4mnx]], [[4mnw]], [[4mnv]] - hUPA catalytic domain (mutant) + bicyclic peptide<br /> | ||
*Coagulase/fibrinolysin | *Coagulase/fibrinolysin | ||
Current revision
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3D structures of plasminogen activator
Updated on 24-November-2019
References
- ↑ Sodeinde OA, Sample AK, Brubaker RR, Goguen JD. Plasminogen activator/coagulase gene of Yersinia pestis is responsible for degradation of plasmid-encoded outer membrane proteins. Infect Immun. 1988 Oct;56(10):2749-52. PMID:2843471
- ↑ . Tissue plasminogen activator for acute ischemic stroke. The National Institute of Neurological Disorders and Stroke rt-PA Stroke Study Group. N Engl J Med. 1995 Dec 14;333(24):1581-7. PMID:7477192 doi:http://dx.doi.org/10.1056/NEJM199512143332401
