1ewj

<StructureSection load='1ewj' size='340' side='right' caption='[[1ewj]], [[Resolution|resolution]] 2.50&Aring;' scene=''>

<table><tr><td colspan='2'>[[1ewj]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWJ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qto|1qto]], [[1byl|1byl]], [[1ecs|1ecs]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ewj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ewj OCA], [http://pdbe.org/1ewj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ewj RCSB], [http://www.ebi.ac.uk/pdbsum/1ewj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ewj ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/BLE_KLEPN BLE_KLEPN]] Binding protein with a strong affinity to the bleomycin family of antibiotics. Binds to CL990; an antimitotic-antibiotic compound.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ewj_consurf.spt"</scriptWhenChecked>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

The transposon Tn5 carries a gene designated ble that confers resistance to bleomycin (Bm). In this study, we determined the x-ray crystal structures of the ble gene product, designated BLMT, uncomplexed and complexed with Bm at 1.7 and 2.5 A resolution, respectively. The structure of BLMT is a dimer with two Bm-binding pockets composed of two large concavities and two long grooves. This crystal structure of BLMT complexed with Bm gives a precise mode for binding of the antibiotic to BLMT. The conformational change of BLMT generated by binding to Bm occurs at a beta-turn composed of the residues from Gln(97) to Thr(102). Crystallographic analysis of Bm bound to BLMT shows that two thiazolium rings of the bithiazole moiety are in the trans conformation. The axial ligand, which binds a metal ion, seems to be the primary amine in the beta-aminoalanine moiety. This report, which is the first with regard to the x-ray crystal structure of Bm, shows that the bithiazole moiety of Bm is far from the metal-binding domain. That is, Bm complexed with BLMT takes a more extended form than the drug complexed with DNA.

Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin.,Maruyama M, Kumagai T, Matoba Y, Hayashida M, Fujii T, Hata Y, Sugiyama M J Biol Chem. 2001 Mar 30;276(13):9992-9. Epub 2000 Dec 29. PMID:11134052<ref>PMID:11134052</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 1ewj" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Hata, Y]]

[[Category: Kumagai, T]]

[[Category: Maruyama, M]]

[[Category: Matoba, Y]]

[[Category: Sugiyama, M]]

[[Category: Antibiotic inhibitor]]

[[Category: Antibiotics resistance]]

[[Category: Homodimer]]