1wj9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:31, 13 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1wj9.jpg|left|200px]]
 
-
{{Structure
+
==Crystal structure of a CRISPR-associated protein from thermus thermophilus==
-
|PDB= 1wj9 |SIZE=350|CAPTION= <scene name='initialview01'>1wj9</scene>, resolution 1.90&Aring;
+
<StructureSection load='1wj9' size='340' side='right'caption='[[1wj9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1wj9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WJ9 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wj9 OCA], [https://pdbe.org/1wj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wj9 RCSB], [https://www.ebi.ac.uk/pdbsum/1wj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wj9 ProSAT], [https://www.topsan.org/Proteins/RSGI/1wj9 TOPSAN]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wj9 OCA], [http://www.ebi.ac.uk/pdbsum/1wj9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wj9 RCSB]</span>
+
[https://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/1wj9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wj9 ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal structure of a CRISPR-associated protein from thermus thermophilus'''
+
==See Also==
-
 
+
*[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]]
-
 
+
*[[CRISPR-Cas|CRISPR-Cas]]
-
==Overview==
+
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-
We have determined the crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 at 1.9 A resolution. This protein is a member of the Escherichia coli ygcH sequence family, which contains approximately 15 sequence homologs of bacterial origin. These homologs have a high isoelectric point. The crystal structure reveals that TTHB192 consists of two independently folded domains, and that each domain exhibits a ferredoxin-like fold with a four-stranded antiparallel beta-sheet packed on one side by alpha-helices. These two tandem domains face each other to generate a beta-sheet platform. TTHB192 displays overall structural similarity to Sex-lethal protein and poly(A)-binding protein fragments. These proteins have RNA binding activity which is supported by a beta-sheet platform formed by two tandem repeats of an RNA recognition motif domain with signature sequence motifs on the beta-sheet surface. Although TTHB192 does not have the same signature sequence motif as the RNA recognition motif domain, the presence of an evolutionarily conserved basic patch on the beta-sheet platform could be functionally relevant for nucleic acid-binding. This report shows that TTHB192 and its sequence homologs adopt an RNA recognition motif-like domain and provides the first testable functional hypothesis for this protein family.
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
1WJ9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WJ9 OCA].
+
</StructureSection>
-
 
+
[[Category: Large Structures]]
-
==Reference==
+
-
Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain., Ebihara A, Yao M, Masui R, Tanaka I, Yokoyama S, Kuramitsu S, Protein Sci. 2006 Jun;15(6):1494-9. Epub 2006 May 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16672237 16672237]
+
-
[[Category: Protein complex]]
+
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
-
[[Category: Ebihara, A.]]
+
[[Category: Ebihara A]]
-
[[Category: Kuramitsu, S.]]
+
[[Category: Kuramitsu S]]
-
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
+
[[Category: Yao M]]
-
[[Category: Yao, M.]]
+
[[Category: Yokoyama S]]
-
[[Category: Yokoyama, S.]]
+
-
[[Category: alpha and beta protein]]
+
-
[[Category: riken structural genomics/proteomics initiative]]
+
-
[[Category: rsgi]]
+
-
[[Category: structural genomic]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:37:02 2008''
+

Current revision

Crystal structure of a CRISPR-associated protein from thermus thermophilus

PDB ID 1wj9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wj9"
Personal tools