5t0o

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==Crystal Structure of a membrane protein==
==Crystal Structure of a membrane protein==
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<StructureSection load='5t0o' size='340' side='right' caption='[[5t0o]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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<StructureSection load='5t0o' size='340' side='right'caption='[[5t0o]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5t0o]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T0O FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5t0o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T0O FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t0o OCA], [http://pdbe.org/5t0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t0o RCSB], [http://www.ebi.ac.uk/pdbsum/5t0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t0o ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t0o OCA], [https://pdbe.org/5t0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t0o RCSB], [https://www.ebi.ac.uk/pdbsum/5t0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t0o ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8RTE4_CAMJU Q8RTE4_CAMJU]
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Resistance-nodulation-cell division efflux pumps are integral membrane proteins that catalyze the export of substrates across cell membranes. Within the hydrophobe-amphiphile efflux subfamily, these resistance-nodulation-cell division proteins largely form trimeric efflux pumps. The drug efflux process has been proposed to entail a synchronized motion between subunits of the trimer to advance the transport cycle, leading to the extrusion of drug molecules. Here we use X-ray crystallography and single-molecule fluorescence resonance energy transfer imaging to elucidate the structures and functional dynamics of the Campylobacter jejuni CmeB multidrug efflux pump. We find that the CmeB trimer displays a very unique conformation. A direct observation of transport dynamics in individual CmeB trimers embedded in membrane vesicles indicates that each CmeB subunit undergoes conformational transitions uncoordinated and independent of each other. On the basis of our findings and analyses, we propose a model for transport mechanism where CmeB protomers function independently within the trimer.Multidrug efflux pumps significantly contribute for bacteria resistance to antibiotics. Here the authors present the structure of Campylobacter jejuni CmeB pump combined with functional FRET assays to propose a transport mechanism where each CmeB protomers is functionally independent from the trimer.
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Structures and transport dynamics of a Campylobacter jejuni multidrug efflux pump.,Su CC, Yin L, Kumar N, Dai L, Radhakrishnan A, Bolla JR, Lei HT, Chou TH, Delmar JA, Rajashankar KR, Zhang Q, Shin YK, Yu EW Nat Commun. 2017 Aug 1;8(1):171. doi: 10.1038/s41467-017-00217-z. PMID:28761097<ref>PMID:28761097</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5t0o" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Su, C C]]
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[[Category: Campylobacter jejuni]]
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[[Category: Yu, E W]]
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[[Category: Large Structures]]
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[[Category: Membrane protein]]
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[[Category: Su C-C]]
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[[Category: Transmembrane protein]]
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[[Category: Yu EW]]

Current revision

Crystal Structure of a membrane protein

PDB ID 5t0o

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