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| | ==SOLUTION STRUCTURE OF PONERATOXIN== | | ==SOLUTION STRUCTURE OF PONERATOXIN== |
| - | <StructureSection load='1g92' size='340' side='right' caption='[[1g92]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1g92' size='340' side='right'caption='[[1g92]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1g92]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G92 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G92 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1g92]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paraponera_clavata Paraponera clavata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G92 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G92 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g92 OCA], [http://pdbe.org/1g92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g92 RCSB], [http://www.ebi.ac.uk/pdbsum/1g92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g92 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g92 OCA], [https://pdbe.org/1g92 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g92 RCSB], [https://www.ebi.ac.uk/pdbsum/1g92 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g92 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/POTX_PARCV POTX_PARCV]] Blocks nicotinic synaptic transmission in insect CNS and affects voltage-gated sodium channels (Nav). It is a strong, but very slowly acting agonist for smooth muscles and its blocks synaptic transmission in the insect CNS in a concentration-dependent manner and depolarizes giant interneurons. Also affects neuromuscular transmission in rat tissue. | + | [https://www.uniprot.org/uniprot/TX1A_PARCV TX1A_PARCV] Blocks nicotinic synaptic transmission in insect CNS and affects voltage-gated sodium channels (Nav). It is a strong, but very slowly acting agonist for smooth muscles and its blocks synaptic transmission in the insect CNS in a concentration-dependent manner and depolarizes giant interneurons. Also affects neuromuscular transmission in rat tissue.<ref>PMID:12097919</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bailly, I]] | + | [[Category: Large Structures]] |
| - | [[Category: Chroboczek, J]] | + | [[Category: Paraponera clavata]] |
| - | [[Category: Dublet, B]] | + | [[Category: Bailly I]] |
| - | [[Category: Fender, P]] | + | [[Category: Chroboczek J]] |
| - | [[Category: Ferber, M L]] | + | [[Category: Dublet B]] |
| - | [[Category: Gout, E]] | + | [[Category: Fender P]] |
| - | [[Category: Michalik, J]] | + | [[Category: Ferber ML]] |
| - | [[Category: Poznanski, J]] | + | [[Category: Gout E]] |
| - | [[Category: Szolajska, E]] | + | [[Category: Michalik J]] |
| - | [[Category: Neurotoxin]]
| + | [[Category: Poznanski J]] |
| - | [[Category: Sodium channel inhibitor]]
| + | [[Category: Szolajska E]] |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
TX1A_PARCV Blocks nicotinic synaptic transmission in insect CNS and affects voltage-gated sodium channels (Nav). It is a strong, but very slowly acting agonist for smooth muscles and its blocks synaptic transmission in the insect CNS in a concentration-dependent manner and depolarizes giant interneurons. Also affects neuromuscular transmission in rat tissue.[1]
Publication Abstract from PubMed
Poneratoxin is a small neuropeptide found in the venom of the ant Paraponera clavata. It is stored in the venom reservoir as an inactive 25-residue peptide. Here we describe both chemically synthesized poneratoxin and poneratoxin obtained by expression in insect cells. When expressed in insect cells, poneratoxin was observed attached to cell membranes. Both synthetic and recombinant ponerotoxins were soluble below pH 4.5. The structure of synthetic poneratoxin was characterized by circular dichroism and solved by nuclear magnetic resonance. In an environment imitating a lipid bilayer, at pH within the range of insect hemolymph, synthetic poneratoxin has a V shape, with two alpha-helices connected by a beta-turn. Insect larvae were paralyzed by injection of either of the purified toxins, with the recombinant one acting faster. The recombinant toxin-producing baculovirus reduced the average survival time of the insect host by 25 h compared with unmodified virus. Mass spectrometry analysis showed that the recombinant toxin has an N-terminal 21-residue extension, possibly improving its stability and/or stabilizing the membrane-bound state. The potential use of poneratoxin for the construction of biological insecticide is discussed.
Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide.,Szolajska E, Poznanski J, Ferber ML, Michalik J, Gout E, Fender P, Bailly I, Dublet B, Chroboczek J Eur J Biochem. 2004 Jun;271(11):2127-36. PMID:15153103[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hendrich AB, Mozrzymas JW, Konopińska D, Scuka M. The effect of poneratoxin on neuromuscular transmission in the rat diaphragm. Cell Mol Biol Lett. 2002;7(2):195-202 PMID:12097919
- ↑ Szolajska E, Poznanski J, Ferber ML, Michalik J, Gout E, Fender P, Bailly I, Dublet B, Chroboczek J. Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide. Eur J Biochem. 2004 Jun;271(11):2127-36. PMID:15153103 doi:http://dx.doi.org/10.1111/j.1432-1033.2004.04128.x
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