1g3v

<StructureSection load='1g3v' size='340' side='right' caption='[[1g3v]], [[Resolution|resolution]] 3.10&Aring;' scene=''>

<table><tr><td colspan='2'>[[1g3v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G3V FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g3v OCA], [http://pdbe.org/1g3v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g3v RCSB], [http://www.ebi.ac.uk/pdbsum/1g3v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g3v ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The aim of this study was to clarify whether Ni2+ ions could bind to guanine bases in a standard B-DNA duplex and eventually induce a B--&gt;Z transition. We have determined by X-ray crystallography at 3.1 A resolution the structure of the alternating deoxynucleotide d(CGTGTACACG), which contains both internal and terminal guanines. The duplex is in the B form. It is shown that nickel ions bind selectively to the N7 atom of guanine 10, which is in an extra-helical position, and guanine 2, which is in the terminal position of the duplex. It does not bind to guanine 4, which lies within a standard B-DNA tract. This simple but unambiguous result proves that nickel ions select between different guanines via steric accessibility. Guanine-Ni2+-guanine bridges among symmetry-related duplexes have also been found. These bridges may explain why Ni2+ ions may act either as a precipitant or a renaturing agent for DNA under certain conditions. The biochemical interaction of nickel with DNA can thus be related to its capacity to specifically bind to B-DNA regions with exposed guanines. Also, from the structural point of view, we have found a terminal cytosine, which forms a C.G:C reverse-Hoogsteen triple structure with a base pair of a neighbor duplex. This type of triplet is seldom found and is here described for the first time for a DNA structure.

Nickel-guanine interactions in DNA: crystal structure of nickel-d[CGTGTACACG]2.,Abrescia NA, Huynh-Dinh T, Subirana JA J Biol Inorg Chem. 2002 Jan;7(1-2):195-9. Epub 2001 Aug 25. PMID:11862555<ref>PMID:11862555</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1g3v" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Abrescia, N G.A]]

[[Category: Subirana, J A]]

[[Category: B-dna]]

[[Category: Nickel binding]]