Poly(A) Polymerase

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<StructureSection load='' size='450' side='right' caption='Structure of Yeast Poly(A) Polymerase with ATP, Mg+2 ion (green) and oligo(A) (PDB entry [[2q66]])' scene='Proteopedia:Main_page_develop/2q66_initial/3'>
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<StructureSection load='' size='350' side='right' caption='Structure of Yeast Poly(A) Polymerase with ATP, Mg+2 ion (green) and oligo(A) (PDB entry [[2q66]])' scene='Proteopedia:Main_page_develop/2q66_initial/3'>
[[Image:2q66.png|left|170px]]
[[Image:2q66.png|left|170px]]
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__TOC__
==Function==
==Function==
[[Poly(A) Polymerase]] (polynucleotide adenylytransferase, [[EC Number|EC]] 2.7.7.19) is the enzyme responsible for adding a polyadenine tail to the 3' end of a nascent pre-mRNA transcript. Its substrates are ATP and RNA. The poly(A) tail that poly-A polymerase adds to the 3' end of the pre-mRNA transcript is important for nuclear export, translation and stability of the mRNA.
[[Poly(A) Polymerase]] (polynucleotide adenylytransferase, [[EC Number|EC]] 2.7.7.19) is the enzyme responsible for adding a polyadenine tail to the 3' end of a nascent pre-mRNA transcript. Its substrates are ATP and RNA. The poly(A) tail that poly-A polymerase adds to the 3' end of the pre-mRNA transcript is important for nuclear export, translation and stability of the mRNA.
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*'''Star-PAP''' selects mRNA targets for polyadenylation<ref>PMID:34576144</ref>.
==Determinants of ATP Recognition==
==Determinants of ATP Recognition==
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</blockquote>
</blockquote>
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</StructureSection>
 
==3D structures of poly(A) polymerase==
==3D structures of poly(A) polymerase==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[Poly(A) polymerase 3D structures]]
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Poly(A) polymerase
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</StructureSection>
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**[[3aqk]], [[3aql]], [[3aqm]], [[3aqn]] – PAP residues 18-431 – ''Escherichia coli''<BR />
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**[[2hhp]], [[2o1p]] – yPAP - yeast<BR />
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**[[1q79]], [[1f5a]] - bPAP-α - bovine<br />
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**[[4lt6]] – hPAP-Υ – human<br />
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**[[3pq1]] – hPAP<br />
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**[[3owg]] – VvPAP catalytic subunit – Vaccinia virus<br />
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**[[3wfo]], [[3wfp]], [[4wby]], [[4wbz]] – AaPAP – ''Aquifex aeolicus''<br />
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*Poly(A) polymerase complex with RNA
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**[[3er8]], [[3er9]], [[3erc]] – VvPAP VP55 + RNA<br />
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**[[1av6]] - VvPAP VP39 + SAH + RNA<br />
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**[[3wfq]], [[3wfr]], [[3wfs]], [[4wc3]], [[4wc4]] [[4wc5]], [[4wc6]], [[4wc7]], [[4x0a]]. [[4x0b]] - AaPAP + RNA<br />
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**[[4wc2]] - AaPAP + RNA + ATP analog<br />
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*Poly(A) polymerase other complexes
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**[[2ga9]] - VvPAP VP55 + VP39 + ATP<BR />
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**[[2gaf]] - VvPAP VP55 (mutant) + VP39<BR />
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**[[1jtf]], [[2vp3]] - VvPAP VP39 residues 1-307 (mutant) + M7GPPPG + SAH<BR />
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**[[1b42]], [[1bky]], [[1eqa]], [[3mag]], [[3mct]], [[4dcg]], [[1av6]], [[1p39]], [[1v39]]. [[1jsz]] - VvPAP VP39 residues 1-297 (mutant) + purine derivative + SAH<BR />
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**[[1vp3]] - VvPAP VP39 + SAH<BR />
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**[[1vp9]], [[1eam]], [[1jte]] - VvPAP VP39 (mutant) + SAH<BR />
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**[[1vpt]] - VvPAP VP39 (mutant) + SAM<BR />
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**[[3c66]] - yPAP residues 1-526 + Fip1 peptide <BR />
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**[[2q66]] – yPAP + polyA + ATP<BR />
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**[[1fa0]] - yPAP + Mn + ATP<br />
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**[[1q78]] – bPAP-α + Mg + ATP<BR />
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**[[4wc0]], [[4wc1]] - AaPAP + nucleotide<br />
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*Speckle targeted PIP5K1A-regulated poly(A) polymerase – STAR-PAP
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**[[5wu1]], [[5wu5]], [[5wu6]] – hSTAR-PAP (mutant)<br />
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**[[5wu2]], [[5wu3]], [[5wu4]] – hSTAR-PAP (mutant) + UTP<br />
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}}
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Note: the regulatory subunit of Vaccinia virus Poly(A) polymerase is called '''VP39''' and the catalytic subunit name is '''VP55'''
Note: the regulatory subunit of Vaccinia virus Poly(A) polymerase is called '''VP39''' and the catalytic subunit name is '''VP55'''
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==Reference==
==Reference==
Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis., Balbo PB, Bohm A, Structure. 2007 Sep;15(9):1117-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17850751 17850751]
Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis., Balbo PB, Bohm A, Structure. 2007 Sep;15(9):1117-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17850751 17850751]
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]
[[Category: Polynucleotide adenylyltransferase]]
[[Category: Polynucleotide adenylyltransferase]]

Current revision

Structure of Yeast Poly(A) Polymerase with ATP, Mg+2 ion (green) and oligo(A) (PDB entry 2q66)

Drag the structure with the mouse to rotate

Note: the regulatory subunit of Vaccinia virus Poly(A) polymerase is called VP39 and the catalytic subunit name is VP55

Additional Resources

For additional information, see: Translation
For additional examples of transferases, see: Transferase

Reference

Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis., Balbo PB, Bohm A, Structure. 2007 Sep;15(9):1117-31. PMID:17850751

  1. ↑ Koshre GR, Shaji F, Mohanan NK, Mohan N, Ali J, Laishram RS. Star-PAP RNA Binding Landscape Reveals Novel Role of Star-PAP in mRNA Metabolism That Requires RBM10-RNA Association. Int J Mol Sci. 2021 Sep 15;22(18):9980. PMID:34576144 doi:10.3390/ijms22189980
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