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5w3j

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(New page: ==Yeast microtubule stabilized with Taxol assembled from mutated tubulin== <StructureSection load='5w3j' size='340' side='right' caption='5w3j, resolution 4.00&Aring;' ...)
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==Yeast microtubule stabilized with Taxol assembled from mutated tubulin==
==Yeast microtubule stabilized with Taxol assembled from mutated tubulin==
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<StructureSection load='5w3j' size='340' side='right' caption='[[5w3j]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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<SX load='5w3j' size='340' side='right' viewer='molstar' caption='[[5w3j]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5w3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_(strain_atcc_204508_/_s288c) Saccharomyces cerevisiae (strain atcc 204508 / s288c)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5w3j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W3J FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w3f|5w3f]], [[5w3h|5w3h]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3j OCA], [http://pdbe.org/5w3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3j RCSB], [http://www.ebi.ac.uk/pdbsum/5w3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3j ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3j OCA], [https://pdbe.org/5w3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w3j RCSB], [https://www.ebi.ac.uk/pdbsum/5w3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3j ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TBA1_YEAST TBA1_YEAST]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB_YEAST TBB_YEAST]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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[https://www.uniprot.org/uniprot/TBA1_YEAST TBA1_YEAST] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microtubules are polymers of alphabeta-tubulin heterodimers essential for all eukaryotes. Despite sequence conservation, there are significant structural differences between microtubules assembled in vitro from mammalian or budding yeast tubulin. Yeast MTs were not observed to undergo compaction at the interdimer interface as seen for mammalian microtubules upon GTP hydrolysis. Lack of compaction might reflect slower GTP hydrolysis or a different degree of allosteric coupling in the lattice. The microtubule plus end-tracking protein Bim1 binds yeast microtubules both between alphabeta-tubulin heterodimers, as seen for other organisms, and within tubulin dimers, but binds mammalian tubulin only at interdimer contacts. At the concentrations used in cryo-electron microscopy, Bim1 causes the compaction of yeast microtubules and induces their rapid disassembly. Our studies demonstrate structural differences between yeast and mammalian microtubules that likely underlie their differing polymerization dynamics. These differences may reflect adaptations to the demands of different cell size or range of physiological growth temperatures.
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Structural differences between yeast and mammalian microtubules revealed by cryo-EM.,Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E J Cell Biol. 2017 Sep 4;216(9):2669-2677. doi: 10.1083/jcb.201612195. Epub 2017, Jun 26. PMID:28652389<ref>PMID:28652389</ref>
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==See Also==
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5w3j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Geyer, E A]]
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[[Category: Large Structures]]
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[[Category: Howes, S C]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Kellogg, E H]]
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[[Category: Geyer EA]]
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[[Category: LaFrance, B]]
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[[Category: Howes SC]]
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[[Category: Nogales, E]]
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[[Category: Kellogg EH]]
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[[Category: Rice, L M]]
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[[Category: LaFrance B]]
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[[Category: Westermann, S]]
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[[Category: Nogales E]]
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[[Category: Zhang, R]]
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[[Category: Rice LM]]
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[[Category: Cytoskeleton]]
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[[Category: Westermann S]]
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[[Category: Hydrolase]]
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[[Category: Zhang R]]
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[[Category: Tubulin]]
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Current revision

Yeast microtubule stabilized with Taxol assembled from mutated tubulin

5w3j, resolution 4.00Å

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