Pyridoxine 5'-phosphate oxidase

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<StructureSection load='' size='400' side='right' scene='48/486466/Cv/1' caption='Pyridoxine 5-phosphate oxidase dimer complex with pyridoxine 5-phosphate, [[2aq6]]' >
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<StructureSection load='' size='350' side='right' scene='48/486466/Cv/1' caption='Pyridoxine 5-phosphate oxidase dimer complex with pyridoxine 5-phosphate, [[2aq6]]' >
== Function ==
== Function ==
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'''Pyridoxine 5’-phosphate oxidase''' (PNPO) catalyzes the oxidation of pyridoxamine-phosphate by molecular oxygen producing pyridoxal 5’-phosphate, ammonia and hydrogen peroxide. Pyridoxal 5’-phosphate (PLP), commonly known as vitamin B6, is a biological essential cofactor. PNPO catalyzes the last step in vitamin B6 metabolism. PNPO uses FMN as cofactor<ref>PMID:12686112</ref>.
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'''Pyridoxine 5’-phosphate oxidase''' or '''Pyridoxamine 5’-phosphate oxidase''' (PNPO) catalyzes the oxidation of pyridoxamine-phosphate by molecular oxygen producing pyridoxal 5’-phosphate, ammonia and hydrogen peroxide. Pyridoxal 5’-phosphate (PLP), commonly known as vitamin B6, is a biological essential cofactor. PNPO catalyzes the last step in vitamin B6 metabolism. PNPO uses FMN as cofactor<ref>PMID:12686112</ref>.
== Disease ==
== Disease ==
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== Structural highlights ==
== Structural highlights ==
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The active site of PNPO shows the product of PNPO catalysis - <scene name='48/486466/Cv/3'>PLP - interacting with both subunits of the enzyme</scene><ref>PMID:16239726</ref>. Water molecules shown as red spheres.
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The active site of PNPO shows the product of PNPO catalysis - <scene name='48/486466/Cv/4'>PLP - interacting with both subunits of the enzyme</scene><ref>PMID:16239726</ref>. Water molecules are shown as red spheres.
</StructureSection>
</StructureSection>
==3D structures of pyridoxine 5'-phosphate oxidase==
==3D structures of pyridoxine 5'-phosphate oxidase==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[1dnl]], [[1g76]], [[1wv4]] – EcPNPO – ''Escherichia coli''<br />
 
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[[1g76]], [[1g77]], [[1g78]], [[1g79]], [[1jnw]] – EcPPO + PLP<br />
 
[[1nrg]] - hPNPO + PLP – human<br />
[[1nrg]] - hPNPO + PLP – human<br />
[[3hy8]] - hPNPO (mutant) + PLP<br />
[[3hy8]] - hPNPO (mutant) + PLP<br />
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[[6h00]] – hPPO (mutant) + FMN<br />
[[2aq6]] - MtPNPO + PLP – ''Mycobacterium tuberculosis''<br />
[[2aq6]] - MtPNPO + PLP – ''Mycobacterium tuberculosis''<br />
[[1y30]] - MtPNPO + FMN<br />
[[1y30]] - MtPNPO + FMN<br />
[[1xxo]] - MtPNPO<br />
[[1xxo]] - MtPNPO<br />
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[[1dnl]], [[1g76]], [[1wv4]] – EcPNPO – ''Escherichia coli''<br />
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[[1g76]], [[1g77]], [[1g78]], [[1g79]], [[1jnw]] – EcPPO + PLP<br />
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[[6ylz]] – EcPNPO (mutant) + FMN + Pi<br />
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[[6ymh]] – EcPNPO (mutant) + FMN + PLP<br />
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[[4hmw]] – BlPNPO + FMN – ''Burkholderia lata''<br />
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[[4hmx]] – BlPNPO + FMN + phenazine carboxylic acid<br />
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[[4hms]] – PfPNPO + FMN – ''Pseudomonas fluorescense''<br />
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[[4hmt]], [[4hmu]], [[4hmv]] – PfPNPO + FMN + phenazine carboxylic acid <br />
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Pyridoxine 5-phosphate oxidase dimer complex with pyridoxine 5-phosphate, 2aq6

Drag the structure with the mouse to rotate

3D structures of pyridoxine 5'-phosphate oxidase

Updated on 08-February-2022

1nrg - hPNPO + PLP – human
3hy8 - hPNPO (mutant) + PLP
6h00 – hPPO (mutant) + FMN
2aq6 - MtPNPO + PLP – Mycobacterium tuberculosis
1y30 - MtPNPO + FMN
1xxo - MtPNPO
1dnl, 1g76, 1wv4 – EcPNPO – Escherichia coli
1g76, 1g77, 1g78, 1g79, 1jnw – EcPPO + PLP
6ylz – EcPNPO (mutant) + FMN + Pi
6ymh – EcPNPO (mutant) + FMN + PLP
4hmw – BlPNPO + FMN – Burkholderia lata
4hmx – BlPNPO + FMN + phenazine carboxylic acid
4hms – PfPNPO + FMN – Pseudomonas fluorescense
4hmt, 4hmu, 4hmv – PfPNPO + FMN + phenazine carboxylic acid

References

  1. ↑ di Salvo ML, Safo MK, Musayev FN, Bossa F, Schirch V. Structure and mechanism of Escherichia coli pyridoxine 5'-phosphate oxidase. Biochim Biophys Acta. 2003 Apr 11;1647(1-2):76-82. PMID:12686112
  2. ↑ Mills PB, Camuzeaux SS, Footitt EJ, Mills KA, Gissen P, Fisher L, Das KB, Varadkar SM, Zuberi S, McWilliam R, Stodberg T, Plecko B, Baumgartner MR, Maier O, Calvert S, Riney K, Wolf NI, Livingston JH, Bala P, Morel CF, Feillet F, Raimondi F, Del Giudice E, Chong WK, Pitt M, Clayton PT. Epilepsy due to PNPO mutations: genotype, environment and treatment affect presentation and outcome. Brain. 2014 May;137(Pt 5):1350-60. doi: 10.1093/brain/awu051. Epub 2014 Mar 18. PMID:24645144 doi:http://dx.doi.org/10.1093/brain/awu051
  3. ↑ Biswal BK, Cherney MM, Wang M, Garen C, James MN. Structures of Mycobacterium tuberculosispyridoxine 5'-phosphate oxidase and its complexes with flavin mononucleotide and pyridoxal 5'-phosphate. Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1492-9. Epub 2005, Oct 19. PMID:16239726 doi:10.1107/S0907444905026673

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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