1wvl

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[[Image:1wvl.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer==
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|PDB= 1wvl |SIZE=350|CAPTION= <scene name='initialview01'>1wvl</scene>, resolution 2.6&Aring;
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<StructureSection load='1wvl' size='340' side='right'caption='[[1wvl]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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<table><tr><td colspan='2'>[[1wvl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WVL FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [https://pdbe.org/1wvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB], [https://www.ebi.ac.uk/pdbsum/1wvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvl ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1azp|1AZP]], [[1wd0|1WD0]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [http://www.ebi.ac.uk/pdbsum/1wvl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB]</span>
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[https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]
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}}
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties.
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'''Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer'''
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Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates.,Wu SW, Ko TP, Chou CC, Wang AH Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219<ref>PMID:16028219</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1wvl" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties.
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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==About this Structure==
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== References ==
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1WVL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates., Wu SW, Ko TP, Chou CC, Wang AH, Proteins. 2005 Sep 1;60(4):617-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16028219 16028219]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
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[[Category: Wang, A H.]]
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[[Category: Wang AH]]
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[[Category: Wu, S W.]]
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[[Category: Wu SW]]
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[[Category: leucine zipper]]
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[[Category: protein engineering]]
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[[Category: synchrotron radiation]]
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[[Category: thermophile]]
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[[Category: x-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:41:34 2008''
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Current revision

Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer

PDB ID 1wvl

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