1wxc

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[[Image:1wxc.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein==
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|PDB= 1wxc |SIZE=350|CAPTION= <scene name='initialview01'>1wxc</scene>, resolution 1.20&Aring;
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<StructureSection load='1wxc' size='340' side='right'caption='[[1wxc]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>
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<table><tr><td colspan='2'>[[1wxc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_castaneoglobisporus Streptomyces castaneoglobisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WXC FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Monophenol_monooxygenase Monophenol monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.18.1 1.14.18.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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|GENE= tyrc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=79261 Streptomyces castaneoglobisporus]), orf378 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=79261 Streptomyces castaneoglobisporus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wxc OCA], [https://pdbe.org/1wxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wxc RCSB], [https://www.ebi.ac.uk/pdbsum/1wxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wxc ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1wx2|1WX2]], [[1wx3|1WX3]], [[1wx4|1WX4]], [[1wx5|1WX5]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wxc OCA], [http://www.ebi.ac.uk/pdbsum/1wxc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wxc RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q83WS1_9ACTN Q83WS1_9ACTN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wx/1wxc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wxc ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein'''
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==See Also==
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*[[Tyrosinase 3D structures|Tyrosinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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At high resolution, we determined the crystal structures of copper-bound and metal-free tyrosinase in a complex with ORF378 designated as a "caddie" protein because it assists with transportation of two CuII ions into the tyrosinase catalytic center. These structures suggest that the caddie protein covers the hydrophobic molecular surface of tyrosinase and interferes with the binding of a substrate tyrosine to the catalytic site of tyrosinase. The caddie protein, which consists of one six-strandedbeta-sheet and one alpha-helix, has no similarity with all proteins deposited into the Protein Data Bank. Although tyrosinase and catechol oxidase are classified into the type 3 copper protein family, the latter enzyme lacks monooxygenase activity. The difference in catalytic activity is based on the structural observations that a large vacant space is present just above the active center of tyrosinase and that one of the six His ligands for the two copper ions is highly flexible. These structural characteristics of tyrosinase suggest that, in the reaction that catalyzes the ortho-hydroxylation of monophenol, one of the two Cu(II) ions is coordinated by the peroxide-originated oxygen bound to the substrate. Our crystallographic study shows evidence that the tyrosinase active center formed by dinuclear coppers is flexible during catalysis.
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[[Category: Large Structures]]
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==About this Structure==
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1WXC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_castaneoglobisporus Streptomyces castaneoglobisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WXC OCA].
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==Reference==
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Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis., Matoba Y, Kumagai T, Yamamoto A, Yoshitsu H, Sugiyama M, J Biol Chem. 2006 Mar 31;281(13):8981-90. Epub 2006 Jan 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16436386 16436386]
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[[Category: Monophenol monooxygenase]]
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[[Category: Protein complex]]
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[[Category: Streptomyces castaneoglobisporus]]
[[Category: Streptomyces castaneoglobisporus]]
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[[Category: Kumagai, T.]]
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[[Category: Kumagai T]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: Yamamoto, A.]]
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[[Category: Yamamoto A]]
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[[Category: Yoshitsu, H.]]
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[[Category: Yoshitsu H]]
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[[Category: binary complex]]
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[[Category: type-3 copper]]
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[[Category: tyrosinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:42:14 2008''
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Current revision

Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein

PDB ID 1wxc

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