5o7t
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5o7t is ON HOLD until Paper Publication Authors: Betz, K., Diederichs, K., Marx, A. Description: Crystal structure of KlenTaq mutant M747K in a clo...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair== | |
| + | <StructureSection load='5o7t' size='340' side='right'caption='[[5o7t]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5o7t]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O7T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7t OCA], [https://pdbe.org/5o7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o7t RCSB], [https://www.ebi.ac.uk/pdbsum/5o7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DPO1_THEAQ DPO1_THEAQ] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The possibility to sequence cytotoxic O(6)-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing. | ||
| - | + | Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.,Betz K, Nilforoushan A, Wyss LA, Diederichs K, Sturla SJ, Marx A Chem Commun (Camb). 2017 Nov 23;53(94):12704-12707. doi: 10.1039/c7cc07173f. PMID:29136072<ref>PMID:29136072</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5o7t" style="background-color:#fffaf0;"></div> |
| - | [[Category: Betz | + | |
| - | [[Category: Marx | + | ==See Also== |
| + | *[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Thermus aquaticus]] | ||
| + | [[Category: Betz K]] | ||
| + | [[Category: Diederichs K]] | ||
| + | [[Category: Marx A]] | ||
Current revision
Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair
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