5odz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5odz is ON HOLD Authors: Lund, B.A., Carlsen, T.J.O., Leiros, H.K.S. Description: CRYSTAL STRUCTURE OF THE BETA-LACTAMASE OXA-163 [[Category: Unrel...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==CRYSTAL STRUCTURE OF THE BETA-LACTAMASE OXA-163== | |
| + | <StructureSection load='5odz' size='340' side='right'caption='[[5odz]], [[Resolution|resolution]] 2.07Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5odz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ODZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5odz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odz OCA], [https://pdbe.org/5odz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5odz RCSB], [https://www.ebi.ac.uk/pdbsum/5odz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5odz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F6KZJ2_ENTCL F6KZJ2_ENTCL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The first crystal structures of the class D beta-lactamases OXA-181 and OXA-245 were determined to 2.05 and 2.20 A resolution, respectively; in addition, the structure of a new crystal form of OXA-163 was resolved to 2.07 A resolution. All of these enzymes are OXA-48-like and have been isolated from different clinical Klebsiella pneumoniae strains and also from other human pathogens such as Pseudomonas aeruginosa and Escherichia coli. Here, enzyme kinetics and thermostability studies are presented, and the new crystal structures are used to explain the observed variations. OXA-245 had the highest melting point (Tm = 55.8 degrees C), as determined by differential scanning calorimetry, compared with OXA-163 (Tm = 49.4 degrees C) and OXA-181 (Tm = 52.6 degrees C). The differences could be explained by the loss of two salt bridges in OXA-163, and an overall decrease in the polarity of the surface of OXA-181 compared with OXA-245. | ||
| - | + | Structure, activity and thermostability investigations of OXA-163, OXA-181 and OXA-245 using biochemical analysis, crystal structures and differential scanning calorimetry analysis.,Lund BA, Thomassen AM, Carlsen TJO, Leiros HKS Acta Crystallogr F Struct Biol Commun. 2017 Oct 1;73(Pt 10):579-587. doi:, 10.1107/S2053230X17013838. Epub 2017 Oct 2. PMID:28994407<ref>PMID:28994407</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Carlsen | + | <div class="pdbe-citations 5odz" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| + | *[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Enterobacter cloacae]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Carlsen TJO]] | ||
| + | [[Category: Leiros HKS]] | ||
| + | [[Category: Lund BA]] | ||
Current revision
CRYSTAL STRUCTURE OF THE BETA-LACTAMASE OXA-163
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