5of1

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'''Unreleased structure'''
 
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The entry 5of1 is ON HOLD
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==The structural versatility of TasA in B. subtilis biofilm formation==
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<StructureSection load='5of1' size='340' side='right'caption='[[5of1]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5of1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OF1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5of1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5of1 OCA], [https://pdbe.org/5of1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5of1 RCSB], [https://www.ebi.ac.uk/pdbsum/5of1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5of1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TASA_BACSU TASA_BACSU] TasA is the major protein component of the biofilm extracellular matrix (PubMed:16430696, PubMed:20080671). It forms amyloid fibers that bind cells together in the biofilm (PubMed:20080671). Exhibits an antibacterial activity against a variety of Gram-positive and Gram-negative bacteria (PubMed:10049401). In laboratory strains, is also involved in proper spore coat assembly (PubMed:10368135).<ref>PMID:10049401</ref> <ref>PMID:10368135</ref> <ref>PMID:16430696</ref> <ref>PMID:20080671</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microorganisms form surface-attached communities, termed biofilms, which can serve as protection against host immune reactions or antibiotics. Bacillus subtilis biofilms contain TasA as major proteinaceous component in addition to exopolysaccharides. In stark contrast to the initially unfolded biofilm proteins of other bacteria, TasA is a soluble, stably folded monomer, whose structure we have determined by X-ray crystallography. Subsequently, we characterized in vitro different oligomeric forms of TasA by NMR, EM, X-ray diffraction, and analytical ultracentrifugation (AUC) experiments. However, by magic-angle spinning (MAS) NMR on live biofilms, a swift structural change toward only one of these forms, consisting of homogeneous and protease-resistant, beta-sheet-rich fibrils, was observed in vivo. Thereby, we characterize a structural change from a globular state to a fibrillar form in a functional prokaryotic system on the molecular level.
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Authors: Roske, Y., Diehl, A., Ball, L., Chowdhury, A., Hiller, M., Moliere, N., Kramer, R., Nagaraj, M., Stoeppler, D., Worth, C.L., Schlegel, B., Leidert, M., Cremer, N., Eisenmenger, F., Lopez, D., Schmieder, P., Heinemann, U., Turgay, K., Akbey, U., Oschkinat, H.
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Structural changes of TasA in biofilm formation of Bacillus subtilis.,Diehl A, Roske Y, Ball L, Chowdhury A, Hiller M, Moliere N, Kramer R, Stoppler D, Worth CL, Schlegel B, Leidert M, Cremer N, Erdmann N, Lopez D, Stephanowitz H, Krause E, van Rossum BJ, Schmieder P, Heinemann U, Turgay K, Akbey U, Oschkinat H Proc Natl Acad Sci U S A. 2018 Mar 12. pii: 1718102115. doi:, 10.1073/pnas.1718102115. PMID:29531041<ref>PMID:29531041</ref>
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Description: The structural versatility of TasA in B. subtilis biofilm formation
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Schmieder, P]]
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<div class="pdbe-citations 5of1" style="background-color:#fffaf0;"></div>
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[[Category: Diehl, A]]
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== References ==
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[[Category: Lopez, D]]
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<references/>
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[[Category: Kramer, R]]
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__TOC__
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[[Category: Turgay, K]]
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</StructureSection>
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[[Category: Ball, L]]
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[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Cremer, N]]
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[[Category: Large Structures]]
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[[Category: Akbey, U]]
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[[Category: Akbey U]]
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[[Category: Moliere, N]]
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[[Category: Ball L]]
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[[Category: Schlegel, B]]
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[[Category: Chowdhury A]]
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[[Category: Oschkinat, H]]
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[[Category: Cremer N]]
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[[Category: Nagaraj, M]]
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[[Category: Diehl A]]
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[[Category: Heinemann, U]]
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[[Category: Eisenmenger F]]
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[[Category: Leidert, M]]
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[[Category: Heinemann U]]
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[[Category: Eisenmenger, F]]
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[[Category: Hiller M]]
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[[Category: Stoeppler, D]]
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[[Category: Kramer R]]
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[[Category: Chowdhury, A]]
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[[Category: Leidert M]]
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[[Category: Roske, Y]]
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[[Category: Lopez D]]
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[[Category: Hiller, M]]
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[[Category: Moliere N]]
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[[Category: Worth, C.L]]
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[[Category: Nagaraj M]]
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[[Category: Oschkinat H]]
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[[Category: Roske Y]]
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[[Category: Schlegel B]]
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[[Category: Schmieder P]]
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[[Category: Stoeppler D]]
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[[Category: Turgay K]]
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[[Category: Worth CL]]

Current revision

The structural versatility of TasA in B. subtilis biofilm formation

PDB ID 5of1

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