5ofs
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5ofs is ON HOLD until Paper Publication Authors: Rothlisberger, U., Bozkurt, E., Hovius, R., Perez, M.A.S., Browning, N.J. Description: X-ray struc...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray structure of a zinc binding GB1 mutant== | |
| + | <StructureSection load='5ofs' size='340' side='right'caption='[[5ofs]], [[Resolution|resolution]] 1.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ofs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OFS FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ofs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ofs OCA], [https://pdbe.org/5ofs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ofs RCSB], [https://www.ebi.ac.uk/pdbsum/5ofs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ofs ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The development of thermostable and solvent-tolerant metalloproteins is a long-sought goal for many applications in synthetic biology and biotechnology. In this work, we were able to engineer a highly thermostable and organic solvent-stable metallo variant of the B1 domain of protein G (GB1) with a tetrahedral zinc binding site reminiscent of the one of thermolysin. Promising candidates were designed computationally by applying a protocol based on classical and first-principles molecular dynamics simulations in combination with genetic algorithm optimization. The most promising of the computationally predicted mutants was expressed and structurally characterized and yielded a highly thermostable protein. The experimental results thus confirm the predictive power of the applied computational protein engineering approach for the de novo design of highly stable metalloproteins. | ||
| - | + | Genetic Algorithm Based Design and Experimental Characterization of a Highly Thermostable Metalloprotein.,Bozkurt E, Perez MAS, Hovius R, Browning NJ, Rothlisberger U J Am Chem Soc. 2018 Mar 26. doi: 10.1021/jacs.7b10660. PMID:29336153<ref>PMID:29336153</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5ofs" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bozkurt | + | |
| - | [[Category: Browning | + | ==See Also== |
| - | [[Category: Hovius | + | *[[Protein G|Protein G]] |
| - | [[Category: Rothlisberger | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptococcus sp. 'group G']] | ||
| + | [[Category: Bozkurt E]] | ||
| + | [[Category: Browning NJ]] | ||
| + | [[Category: Hovius R]] | ||
| + | [[Category: Perez MAS]] | ||
| + | [[Category: Rothlisberger U]] | ||
Current revision
X-ray structure of a zinc binding GB1 mutant
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