5oq3
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5oq3 is ON HOLD until Paper Publication Authors: Bradshaw, W.J., Kirby, J.M., Roberts, A.K., Shone, C.C., Acharya, K.R. Description: High resolutio...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==High resolution structure of the functional region of Cwp19 from Clostridium difficile== | |
| + | <StructureSection load='5oq3' size='340' side='right'caption='[[5oq3]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5oq3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile Clostridioides difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OQ3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oq3 OCA], [https://pdbe.org/5oq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oq3 RCSB], [https://www.ebi.ac.uk/pdbsum/5oq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oq3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/L7PGA3_CLODI L7PGA3_CLODI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clostridium difficile is a burden to health care systems around the world, causing tens of thousands of deaths annually. The S-layer of the bacterium, a layer of protein found of the surface of cells, has received a significant amount of attention over the past two decades as a potential target to combat the growing threat presented by C. difficile infections. The S-layer contains a wide range of proteins, each of which possess three cell wall binding domains, while many also possess a "functional" region. Here, we present the high resolution structure of the functional region of one such protein, Cwp19 along with preliminary functional characterisation of the predicted glycoside hydrolase. Cwp19 has a TIM barrel fold and appears to possess a high degree of substrate selectivity. The protein also exhibits peptidoglycan hydrolase activity an order of magnitude slower than that of lysozyme and is the first member of glycoside hydrolase-like family 10 to be characterised. This research goes some way to understanding the role of Cwp19 in the S-layer of C. difficile. This article is protected by copyright. All rights reserved. | ||
| - | + | The molecular structure of the glycoside hydrolase domain of Cwp19 from Clostridium difficile.,Bradshaw WJ, Kirby JM, Roberts AK, Shone CC, Acharya KR FEBS J. 2017 Oct 30. doi: 10.1111/febs.14310. PMID:29083543<ref>PMID:29083543</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Acharya | + | <div class="pdbe-citations 5oq3" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bradshaw | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Clostridioides difficile]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Acharya KR]] | ||
| + | [[Category: Bradshaw WJ]] | ||
| + | [[Category: Kirby JM]] | ||
| + | [[Category: Roberts AK]] | ||
| + | [[Category: Shone CC]] | ||
Current revision
High resolution structure of the functional region of Cwp19 from Clostridium difficile
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