|
|
| (12 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1xa2.gif|left|200px]] | |
| | | | |
| - | {{Structure
| + | ==Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA).d(TGCGCG) in two crystal forms== |
| - | |PDB= 1xa2 |SIZE=350|CAPTION= <scene name='initialview01'>1xa2</scene>, resolution 1.71Å
| + | <StructureSection load='1xa2' size='340' side='right'caption='[[1xa2]], [[Resolution|resolution]] 1.71Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE+ION'>NCO</scene>
| + | <table><tr><td colspan='2'>[[1xa2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XA2 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa2 OCA], [https://pdbe.org/1xa2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xa2 RCSB], [https://www.ebi.ac.uk/pdbsum/1xa2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa2 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa2 OCA], [http://www.ebi.ac.uk/pdbsum/1xa2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xa2 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA).d(TGCGCG) in two crystal forms'''
| + | [[Category: Gautham N]] |
| - | | + | [[Category: Rajan SS]] |
| - | | + | [[Category: Thiyagarajan S]] |
| - | ==Overview==
| + | |
| - | We report here the crystal structure of the DNA hexamer duplex d(CGCGCA)*d(TGCGCG) at 1.71 A resolution. The crystals, in orthorhombic space group, were grown in the presence of cobalt hexammine, a known inducer of the left-handed Z form of DNA. The interaction of this ion with the DNA helix results in a change of the adenine base from the common amino tautomeric form to the imino tautomer. Consequently the A:T base pair is disrupted from the normal Watson-Crick base pairing to a 'wobble' like base pairing. This change is accommodated easily within the helix, and the helical parameters are those expected for Z-DNA. When the cobalt hexammine concentration is decreased slightly in the crystallization conditions, the duplex crystallizes in a different, hexagonal space group, with two hexamer duplexes in the asymmetric unit. One of these is situated on a crystallographic 6-fold screw axis, leading to disorder. The tautomeric shift is not observed in this space group. We show that the change in inter-helix interactions that lead to the two different space groups probably arise from the small decrease in ion concentration, and consequently disordered positions for the ion.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1XA2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA2 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15534365 15534365]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Gautham, N.]] | + | |
| - | [[Category: Rajan, S S.]] | + | |
| - | [[Category: Thiyagarajan, S.]] | + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: z-dna]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:46:47 2008''
| + | |
