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| - | [[Image:1xam.gif|left|200px]] | |
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| - | {{Structure
| + | ==Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.== |
| - | |PDB= 1xam |SIZE=350|CAPTION= <scene name='initialview01'>1xam</scene>, resolution 1.86Å
| + | <StructureSection load='1xam' size='340' side='right'caption='[[1xam]], [[Resolution|resolution]] 1.86Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE+ION'>NCO</scene>
| + | <table><tr><td colspan='2'>[[1xam]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAM FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xam OCA], [https://pdbe.org/1xam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xam RCSB], [https://www.ebi.ac.uk/pdbsum/1xam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xam ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[1xa2|1XA2]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xam OCA], [http://www.ebi.ac.uk/pdbsum/1xam PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xam RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.'''
| + | [[Category: Gautham N]] |
| - | | + | [[Category: Rajan SS]] |
| - | | + | [[Category: Thiyagarajan S]] |
| - | ==Overview==
| + | |
| - | We report here the crystal structure of the DNA hexamer duplex d(CGCGCA)*d(TGCGCG) at 1.71 A resolution. The crystals, in orthorhombic space group, were grown in the presence of cobalt hexammine, a known inducer of the left-handed Z form of DNA. The interaction of this ion with the DNA helix results in a change of the adenine base from the common amino tautomeric form to the imino tautomer. Consequently the A:T base pair is disrupted from the normal Watson-Crick base pairing to a 'wobble' like base pairing. This change is accommodated easily within the helix, and the helical parameters are those expected for Z-DNA. When the cobalt hexammine concentration is decreased slightly in the crystallization conditions, the duplex crystallizes in a different, hexagonal space group, with two hexamer duplexes in the asymmetric unit. One of these is situated on a crystallographic 6-fold screw axis, leading to disorder. The tautomeric shift is not observed in this space group. We show that the change in inter-helix interactions that lead to the two different space groups probably arise from the small decrease in ion concentration, and consequently disordered positions for the ion.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1XAM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAM OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15534365 15534365]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Gautham, N.]] | + | |
| - | [[Category: Rajan, S S.]] | + | |
| - | [[Category: Thiyagarajan, S.]] | + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: z-dna]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:47:02 2008''
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