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| - | [[Image:1xce.gif|left|200px]] | |
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| - | {{Structure
| + | ==Helica Structure of DNA by Design: The T(GGGG)T Hexad Alignment== |
| - | |PDB= 1xce |SIZE=350|CAPTION= <scene name='initialview01'>1xce</scene>
| + | <StructureSection load='1xce' size='340' side='right'caption='[[1xce]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1xce]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCE FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xce OCA], [https://pdbe.org/1xce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xce RCSB], [https://www.ebi.ac.uk/pdbsum/1xce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xce ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xce OCA], [http://www.ebi.ac.uk/pdbsum/1xce PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xce RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Homo sapiens]] |
| - | | + | [[Category: Large Structures]] |
| - | '''Helica Structure of DNA by Design: The T(GGGG)T Hexad Alignment'''
| + | [[Category: Webba da Silva M]] |
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| - | | + | |
| - | ==Overview==
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| - | A template-based approach was used to design unprecedented architectural motifs into a known DNA framework. The structure formed by the sequence d(GCGGTTGGAT) in 0.1 M Na(+) solution has been determined using molecular dynamics simulations constrained by distance and dihedral restraints derived from NMR experiments. The molecular topology has been previously observed for the sequence d(GCGGTGGAT) (Webba da Silva, M. (2003) Biochemistry 42, 14356-65). Insertion of a single thymine into the double chain reversal formed by the segment GGTGG results in the unprecedented experimental demonstration of a T:(G:G:G:G):T hexad. The bi-stranded hexad results from the pairing alignment of two G(T-G) triads. Each triad results from recognition of the sheared edge of a guanine by the Watson-Crick edge of a thymine of the segment GGTTGG. The alignment is stabilized by base-stacking of the thymine to the sugar pucker of the preceding thymine. The latter is involved in formation of the T:A:A:T tetrad alignment by forming a hydrogen bond with the free amino proton of a Watson-Crick aligned A:A mispair. We have thus established that residues in double chain reversal loops linking juxtaposed tetrads of a quadruplex stem may facilitate formation of yet unknown hydrogen bond alignments. By employing a systematic approach analysis of sequence motifs appearing in double chain reversals, bridging tetrad layers should allow for the prediction of topologies and architectural motifs appearing in biologically relevant genomic regions.
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| - | ==About this Structure==
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| - | 1XCE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCE OCA].
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| - | ==Reference==
| + | |
| - | Experimental demonstration of T:(G:G:G:G):T hexad and T:A:A:T tetrad alignments within a DNA quadruplex stem., Webba da Silva M, Biochemistry. 2005 Mar 15;44(10):3754-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15751952 15751952]
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| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Silva, M Webba da.]] | + | |
| - | [[Category: dna quadruplex]] | + | |
| - | [[Category: hexad]] | + | |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:47:39 2008''
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