1xcr

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[[Image:1xcr.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold==
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|PDB= 1xcr |SIZE=350|CAPTION= <scene name='initialview01'>1xcr</scene>, resolution 1.70&Aring;
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<StructureSection load='1xcr' size='340' side='right'caption='[[1xcr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1xcr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= DKFZp564H1122 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcr OCA], [https://pdbe.org/1xcr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcr RCSB], [https://www.ebi.ac.uk/pdbsum/1xcr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcr ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcr OCA], [http://www.ebi.ac.uk/pdbsum/1xcr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xcr RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CK054_HUMAN CK054_HUMAN] Exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.<ref>PMID:16522806</ref>
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== Evolutionary Conservation ==
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'''Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcr_consurf.spt"</scriptWhenChecked>
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The human protein PTD012 is the longer product of an alternatively spliced gene and was described to be localized in the nucleus. The X-ray structure analysis at 1.7 A resolution of PTD012 through SAD phasing reveals a monomeric protein and a novel fold. The shorter splice form was also studied and appears to be unfolded and non-functional. The structure of PTD012 displays an alphabetabetaalpha four-layer topology. A metal ion residing between the central beta-sheets is partially coordinated by three histidine residues. X-ray absorption near-edge structure (XANES) analysis identifies the PTD012-bound ion as Zn(2+). Tetrahedral coordination of the ion is completed by the carboxylate oxygen atom of an acetate molecule taken up from the crystallization buffer. The binding of Zn(2+) to PTD012 is reminiscent of zinc-containing enzymes such as carboxypeptidase, carbonic anhydrase, and beta-lactamase. Biochemical assays failed to demonstrate any of these enzyme activities in PTD012. However, PTD012 exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1XCR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCR OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcr ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold., Manjasetty BA, Bussow K, Fieber-Erdmann M, Roske Y, Gobom J, Scheich C, Gotz F, Niesen FH, Heinemann U, Protein Sci. 2006 Apr;15(4):914-20. Epub 2006 Mar 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16522806 16522806]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Buessow, K.]]
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[[Category: Buessow K]]
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[[Category: Fieber-Erdmann, M.]]
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[[Category: Fieber-Erdmann M]]
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[[Category: Goetz, F.]]
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[[Category: Goetz F]]
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[[Category: Heinemann, U.]]
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[[Category: Heinemann U]]
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[[Category: Manjasetty, B A.]]
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[[Category: Manjasetty BA]]
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[[Category: Roske, Y.]]
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[[Category: Roske Y]]
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[[Category: acetate buffer]]
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[[Category: splice variant]]
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[[Category: structural genomic]]
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[[Category: zinc-containing fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:47:48 2008''
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Current revision

Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold

PDB ID 1xcr

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