5w3b

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'''Unreleased structure'''
 
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The entry 5w3b is ON HOLD until Paper Publication
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==Crystal structure of mutant CJ YCEI protein (CJ-N182C) with mercuribenzoic acid guest structure==
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<StructureSection load='5w3b' size='340' side='right'caption='[[5w3b]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5w3b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W3B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=MBO:MERCURIBENZOIC+ACID'>MBO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3b OCA], [https://pdbe.org/5w3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w3b RCSB], [https://www.ebi.ac.uk/pdbsum/5w3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q0PB90_CAMJE Q0PB90_CAMJE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (&gt;13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.
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Authors: Huber, T.R., Snow, C.D.
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Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.,Huber TR, McPherson EC, Keating CE, Snow CD Bioconjug Chem. 2017 Dec 20. doi: 10.1021/acs.bioconjchem.7b00668. PMID:29232505<ref>PMID:29232505</ref>
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Description: Crystal structure of mutant CJ YCEI protein (CJ-N182C) with mercuribenzoic acid guest structure
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Huber, T.R]]
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<div class="pdbe-citations 5w3b" style="background-color:#fffaf0;"></div>
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[[Category: Snow, C.D]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Campylobacter jejuni]]
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[[Category: Large Structures]]
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[[Category: Huber TR]]
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[[Category: Snow CD]]

Current revision

Crystal structure of mutant CJ YCEI protein (CJ-N182C) with mercuribenzoic acid guest structure

PDB ID 5w3b

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