1xhp

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[[Image:1xhp.gif|left|200px]]
 
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{{Structure
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==Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae==
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|PDB= 1xhp |SIZE=350|CAPTION= <scene name='initialview01'>1xhp</scene>
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<StructureSection load='1xhp' size='340' side='right'caption='[[1xhp]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[1xhp]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XHP FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xhp OCA], [https://pdbe.org/1xhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xhp RCSB], [https://www.ebi.ac.uk/pdbsum/1xhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xhp ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1sy4|1SY4]], [[1r2p|1R2P]], [[1kxk|1KXK]]
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<div style="background-color:#fffaf0;">
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xhp OCA], [http://www.ebi.ac.uk/pdbsum/1xhp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xhp RCSB]</span>
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== Publication Abstract from PubMed ==
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}}
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'''Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae'''
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==Overview==
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Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site.
Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site.
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==About this Structure==
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U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction.,Sashital DG, Cornilescu G, McManus CJ, Brow DA, Butcher SE Nat Struct Mol Biol. 2004 Dec;11(12):1237-42. Epub 2004 Nov 14. PMID:15543154<ref>PMID:15543154</ref>
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1XHP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XHP OCA].
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==Reference==
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U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction., Sashital DG, Cornilescu G, McManus CJ, Brow DA, Butcher SE, Nat Struct Mol Biol. 2004 Dec;11(12):1237-42. Epub 2004 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15543154 15543154]
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[[Category: Protein complex]]
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[[Category: Butcher, S E.]]
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[[Category: Cornilescu, G.]]
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[[Category: Sashital, D G.]]
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[[Category: internal bulge]]
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[[Category: metal binding site]]
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[[Category: penta-loop]]
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[[Category: rna stem-loop]]
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[[Category: u6 rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:49:47 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1xhp" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Butcher SE]]
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[[Category: Cornilescu G]]
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[[Category: Sashital DG]]

Current revision

Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae

PDB ID 1xhp

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