5w9q

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(New page: '''Unreleased structure''' The entry 5w9q is ON HOLD until Jun 23 2019 Authors: Liu, K., Xu, C., Tempel, W., Walker, J.R., Arrowsmith, C.H., Bountra, C., Edwards, A.M., Min, J., Structu...)
Current revision (14:09, 4 October 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5w9q is ON HOLD until Jun 23 2019
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==Zinc finger region of MBD1 in complex with CpG DNA==
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<StructureSection load='5w9q' size='340' side='right'caption='[[5w9q]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5w9q]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W9Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w9q OCA], [https://pdbe.org/5w9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w9q RCSB], [https://www.ebi.ac.uk/pdbsum/5w9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w9q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MBD1_HUMAN MBD1_HUMAN] Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.<ref>PMID:9207790</ref> <ref>PMID:10454587</ref> <ref>PMID:9774669</ref> <ref>PMID:10648624</ref> <ref>PMID:12711603</ref> <ref>PMID:12665582</ref> <ref>PMID:12697822</ref> <ref>PMID:14610093</ref> <ref>PMID:15327775</ref> <ref>PMID:14555760</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The CXXC domain, first identified as the reader of unmodified CpG dinucleotide, plays important roles in epigenetic regulation by targeting various activities to CpG islands. Here we systematically measured and compared the DNA-binding selectivities of all known human CXXC domains by different binding assays, and complemented the existing function-based classification of human CXXC domains with a classification based on their DNA selectivities. Through a series of crystal structures of CXXC domains with DNA ligands, we unravel the molecular mechanisms of how these CXXC domains, including single CXXC domains and tandem CXXC-PHD domains, recognize distinct DNA ligands, which further supports our classification of human CXXC domains and also provides insights into selective recruitment of chromatin modifiers to their respective targets via CXXC domains recognizing different genomic DNA sequences. Our study facilitates the understanding of the relationship between the DNA-binding specificities of the CXXC proteins and their biological functions.
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Authors: Liu, K., Xu, C., Tempel, W., Walker, J.R., Arrowsmith, C.H., Bountra, C., Edwards, A.M., Min, J., Structural Genomics Consortium (SGC)
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DNA Sequence Recognition of Human CXXC Domains and Their Structural Determinants.,Xu C, Liu K, Lei M, Yang A, Li Y, Hughes TR, Min J Structure. 2017 Dec 16. pii: S0969-2126(17)30396-9. doi:, 10.1016/j.str.2017.11.022. PMID:29276034<ref>PMID:29276034</ref>
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Description: Zinc finger region of MBD1 in complex with CpG DNA
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Min, J]]
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<div class="pdbe-citations 5w9q" style="background-color:#fffaf0;"></div>
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[[Category: Walker, J.R]]
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[[Category: Bountra, C]]
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==See Also==
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[[Category: Tempel, W]]
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*[[Methyl CpG binding protein 3D structures|Methyl CpG binding protein 3D structures]]
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[[Category: Arrowsmith, C.H]]
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== References ==
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[[Category: Xu, C]]
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<references/>
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[[Category: Liu, K]]
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__TOC__
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[[Category: Edwards, A.M]]
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</StructureSection>
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[[Category: Structural Genomics Consortium (Sgc)]]
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Arrowsmith CH]]
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[[Category: Bountra C]]
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[[Category: Edwards AM]]
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[[Category: Liu K]]
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[[Category: Min J]]
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[[Category: Tempel W]]
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[[Category: Walker JR]]
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[[Category: Xu C]]

Current revision

Zinc finger region of MBD1 in complex with CpG DNA

PDB ID 5w9q

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