5xbm

From Proteopedia

(Difference between revisions)

Current revision

Structure of SCARB2-JL2 complex

Structural highlights

5xbm is a 6 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.501Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

SCRB2_HUMAN Unverricht-Lundborg disease;Gaucher disease type 1;Action myoclonus - renal failure syndrome. The disease is caused by mutations affecting the gene represented in this entry. Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.

Function

SCRB2_HUMAN Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.^[1]

Publication Abstract from PubMed

Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving alpha-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.

The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection.,Zhang X, Yang P, Wang N, Zhang J, Li J, Guo H, Yin X, Rao Z, Wang X, Zhang L Protein Cell. 2017 Aug;8(8):590-600. doi: 10.1007/s13238-017-0405-7. Epub 2017, Apr 26. PMID:28447294^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reczek D, Schwake M, Schroder J, Hughes H, Blanz J, Jin X, Brondyk W, Van Patten S, Edmunds T, Saftig P. LIMP-2 is a receptor for lysosomal mannose-6-phosphate-independent targeting of beta-glucocerebrosidase. Cell. 2007 Nov 16;131(4):770-83. PMID:18022370 doi:http://dx.doi.org/10.1016/j.cell.2007.10.018
↑ Zhang X, Yang P, Wang N, Zhang J, Li J, Guo H, Yin X, Rao Z, Wang X, Zhang L. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection. Protein Cell. 2017 Aug;8(8):590-600. doi: 10.1007/s13238-017-0405-7. Epub 2017, Apr 26. PMID:28447294 doi:http://dx.doi.org/10.1007/s13238-017-0405-7

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xbm"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5xbm is ON HOLD
+==Structure of SCARB2-JL2 complex==
+<StructureSection load='5xbm' size='340' side='right'caption='[[5xbm]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5xbm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XBM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.501&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbm OCA], [https://pdbe.org/5xbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xbm RCSB], [https://www.ebi.ac.uk/pdbsum/5xbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbm ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/SCRB2_HUMAN SCRB2_HUMAN] Unverricht-Lundborg disease;Gaucher disease type 1;Action myoclonus - renal failure syndrome. The disease is caused by mutations affecting the gene represented in this entry.  Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
+== Function ==
+[https://www.uniprot.org/uniprot/SCRB2_HUMAN SCRB2_HUMAN] Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.<ref>PMID:18022370</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving alpha-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.
-Authors: Zhang, X., Yang, P., Wang, N., Zhang, J., Li, J., Guo, H., Yin, X., Rao, Z., Wang, X., Zhang, L.
+The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection.,Zhang X, Yang P, Wang N, Zhang J, Li J, Guo H, Yin X, Rao Z, Wang X, Zhang L Protein Cell. 2017 Aug;8(8):590-600. doi: 10.1007/s13238-017-0405-7. Epub 2017, Apr 26. PMID:28447294<ref>PMID:28447294</ref>
-Description: Structure of SCARB2-JL2 complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Yang, P]]
+<div class="pdbe-citations 5xbm" style="background-color:#fffaf0;"></div>
-[[Category: Li, J]]
-[[Category: Rao, Z]]
+==See Also==
-[[Category: Wang, N]]
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
-[[Category: Zhang, X]]
+== References ==
-[[Category: Wang, X]]
+<references/>
-[[Category: Zhang, J]]
+__TOC__
-[[Category: Zhang, L]]
+</StructureSection>
-[[Category: Yin, X]]
+[[Category: Homo sapiens]]
-[[Category: Guo, H]]
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Guo H]]
+[[Category: Li J]]
+[[Category: Rao Z]]
+[[Category: Wang N]]
+[[Category: Wang X]]
+[[Category: Yang P]]
+[[Category: Yin X]]
+[[Category: Zhang J]]
+[[Category: Zhang L]]
+[[Category: Zhang X]]

5xbm

From Proteopedia

Current revision

Structure of SCARB2-JL2 complex

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox