1xne

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[[Image:1xne.jpg|left|200px]]
 
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{{Structure
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==Solution Structure of Pyrococcus furiosus Protein PF0470: The Northeast Structural Genomics Consortium Target PfR14==
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|PDB= 1xne |SIZE=350|CAPTION= <scene name='initialview01'>1xne</scene>
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<StructureSection load='1xne' size='340' side='right'caption='[[1xne]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1xne]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XNE FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xne OCA], [https://pdbe.org/1xne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xne RCSB], [https://www.ebi.ac.uk/pdbsum/1xne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xne ProSAT], [https://www.topsan.org/Proteins/NESGC/1xne TOPSAN]</span></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG4043 COG4043]</span>
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xne OCA], [http://www.ebi.ac.uk/pdbsum/1xne PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xne RCSB]</span>
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[https://www.uniprot.org/uniprot/Q8U3J6_PYRFU Q8U3J6_PYRFU]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Solution Structure of Pyrococcus furiosus Protein PF0470: The Northeast Structural Genomics Consortium Target PfR14'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xn/1xne_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five G-matrix Fourier transform NMR experiments for resonance assignment based on highly resolved 4D and 5D spectral information is acquired in conjunction with a single simultaneous 3D 15N,13C(aliphatic),13C(aromatic)-resolved [1H,1H]-NOESY spectrum providing 1H-1H upper distance limit constraints. The protocol was integrated with methodology for semiautomated data analysis and used to solve eight NMR protein structures of the Northeast Structural Genomics Consortium pipeline. The molecular masses of the hypothetical target proteins ranged from 9 to 20 kDa with an average of approximately 14 kDa. Between 1 and 9 days of instrument time were invested per structure, which is less than approximately 10-25% of the measurement time routinely required to date with conventional approaches. The protocol presented here effectively removes data collection as a bottleneck for high-throughput solution structure determination of proteins up to at least approximately 20 kDa, while concurrently providing spectra that are highly amenable to fast and robust analysis.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xne ConSurf].
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1XNE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNE OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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NMR data collection and analysis protocol for high-throughput protein structure determination., Liu G, Shen Y, Atreya HS, Parish D, Shao Y, Sukumaran DK, Xiao R, Yee A, Lemak A, Bhattacharya A, Acton TA, Arrowsmith CH, Montelione GT, Szyperski T, Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10487-92. Epub 2005 Jul 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16027363 16027363]
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[[Category: Large Structures]]
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[[Category: Pyrococcus furiosus]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Single protein]]
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[[Category: Acton T]]
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[[Category: Acton, T.]]
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[[Category: Liu G]]
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[[Category: Liu, G.]]
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[[Category: Ma L]]
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[[Category: Ma, L.]]
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[[Category: Montelione GT]]
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[[Category: Montelione, G T.]]
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[[Category: Parish D]]
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[[Category: NESG, Northeast Structural Genomics Consortium.]]
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[[Category: Sukumaran D]]
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[[Category: Parish, D.]]
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[[Category: Szyperski T]]
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[[Category: Sukumaran, D.]]
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[[Category: Xiao R]]
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[[Category: Szyperski, T.]]
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[[Category: Xiao, R.]]
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[[Category: alpha and beta protein]]
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[[Category: gft nmr]]
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[[Category: nesg]]
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[[Category: northeast structural genomics consortium]]
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[[Category: pfr14]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:52:03 2008''
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Current revision

Solution Structure of Pyrococcus furiosus Protein PF0470: The Northeast Structural Genomics Consortium Target PfR14

PDB ID 1xne

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