5xxn

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'''Unreleased structure'''
 
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The entry 5xxn is ON HOLD until Paper Publication
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==Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose==
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<StructureSection load='5xxn' size='340' side='right'caption='[[5xxn]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5xxn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XXN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxn OCA], [https://pdbe.org/5xxn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xxn RCSB], [https://www.ebi.ac.uk/pdbsum/5xxn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8A1U1_BACTN Q8A1U1_BACTN]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BT_3567 protein, a putative beta-glucosidase from Bacteroides thetaiotaomicron, exhibits higher activity toward Sop3-5 (Sopn , n: degree of polymerization of beta-1,2-glucooligosaccharides) than toward Sop2 , unlike a known beta-glucosidase from Listeria innocua which predominantly prefers Sop2 . In the complex structure determined by soaking of a D286N mutant crystal with Sop4 , a Sop3 moiety was observed at subsites -1 to +2. The glucose moiety at subsite +2 forms a hydrogen bond with Asn81, which is replaced with Gly in the L. innocua beta-glucosidase. The Km values of the N81G mutant for Sop3-5 are much higher than those of the wild-type, suggesting that Asn81 contributes to the binding to substrates longer than Sop3 .
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Authors: Nakajima, M., Ishiguro, R., Tanaka, N., Abe, K., Maeda, T., Miyanaga, A., Takahashi, Y., Sugimono, N., Nakai, H., Taguchi, H.
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Function and structure relationships of a beta-1,2-glucooligosaccharide-degrading beta-glucosidase.,Ishiguro R, Tanaka N, Abe K, Nakajima M, Maeda T, Miyanaga A, Takahashi Y, Sugimoto N, Nakai H, Taguchi H FEBS Lett. 2017 Dec;591(23):3926-3936. doi: 10.1002/1873-3468.12911. Epub 2017, Nov 24. PMID:29131329<ref>PMID:29131329</ref>
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Description: Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Nakai, H]]
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<div class="pdbe-citations 5xxn" style="background-color:#fffaf0;"></div>
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[[Category: Nakajima, M]]
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[[Category: Sugimono, N]]
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==See Also==
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[[Category: Taguchi, H]]
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*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
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[[Category: Ishiguro, R]]
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== References ==
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[[Category: Takahashi, Y]]
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<references/>
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[[Category: Tanaka, N]]
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__TOC__
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[[Category: Maeda, T]]
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</StructureSection>
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[[Category: Miyanaga, A]]
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[[Category: Bacteroides thetaiotaomicron VPI-5482]]
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[[Category: Abe, K]]
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[[Category: Large Structures]]
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[[Category: Abe K]]
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[[Category: Ishiguro R]]
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[[Category: Maeda T]]
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[[Category: Miyanaga A]]
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[[Category: Nakai H]]
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[[Category: Nakajima M]]
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[[Category: Sugimono N]]
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[[Category: Taguchi H]]
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[[Category: Takahashi Y]]
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[[Category: Tanaka N]]

Current revision

Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose

PDB ID 5xxn

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