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5xyv
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5xyv is ON HOLD until Paper Publication Authors: Yu, B.W., Huang, Y. Description: Crystal structure of drosophila melanogaster Rhino chromoshadow d...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain== | |
| + | <StructureSection load='5xyv' size='340' side='right'caption='[[5xyv]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XYV FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [https://pdbe.org/5xyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [https://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q7JXA8_DROME Q7JXA8_DROME] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level. | ||
| - | + | Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.,Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y EMBO Rep. 2018 Jun 1. pii: embr.201745418. doi: 10.15252/embr.201745418. PMID:29858487<ref>PMID:29858487</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5xyv" style="background-color:#fffaf0;"></div> |
| - | [[Category: Huang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Drosophila melanogaster]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Huang Y]] | ||
| + | [[Category: Yu BW]] | ||
Current revision
Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain
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