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1lgh

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[[Image:1lgh.gif|left|200px]]<br />
 
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<applet load="1lgh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lgh, resolution 2.4&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE LIGHT-HARVESTING COMPLEX II (B800-850) FROM RHODOSPIRILLUM MOLISCHIANUM'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE LIGHT-HARVESTING COMPLEX II (B800-850) FROM RHODOSPIRILLUM MOLISCHIANUM==
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BACKGROUND: The light-harvesting complexes II (LH-2s) are integral, membrane proteins that form ring-like structures, oligomers of alpha, beta-heterodimers, in the photosynthetic membranes of purple bacteria., They contain a large number of chromophores organized optimally for light, absorption and rapid light energy migration. Recently, the structure of, the nonameric LH-2 of Rhodopseudomonas acidophila has been determined; we, report here the crystal structure of the octameric LH-2 from, Rhodospirillum molischianum. The unveiling of similarities and differences, in the architecture of these proteins may provide valuable insight into, the efficient energy transfer mechanisms of bacterial photosynthesis., RESULTS: The crystal structure of LH-2 from Rs. molischianum has been, determined by molecular replacement at 2.4 A resolution using X-ray, diffraction. The crystal structure displays two concentric cylinders of, sixteen membrane-spanning helical subunits, containing two rings of, bacteriochlorophyll-a (BChl-a) molecules. One ring comprises sixteen B850, BChl-as perpendicular to the membrane plane and the other eight B800, BChl-as that are nearly parallel to the membrane plane; eight, membrane-spanning lycopenes (the major carotenoid in this complex) stretch, out between the B800 and B850 BChl-as. The B800 BChl-as exhibit a, different ligation from that of Rps. acidophila (aspartate is the Mg, ligand as opposed to formyl-methionine in Rps. acidophila). CONCLUSIONS:, The light-harvesting complexes from different bacteria assume various ring, sizes. In LH-2 of Rs. molischianum, the Qy transition dipole moments of, neighbouring B850 and B800 BChl-as are nearly parallel to each other, that, is, they are optimally aligned for Foster exciton transfer. Dexter energy, transfer between these chlorophylls is also possible through interactions, mediated by lycopenes and B850 BChl-a phytyl tails; the B800 BChl-a and, one of the two B850 BChl-as associated with each heterodimeric unit are in, van der Waals distance to a lycopene, such that singlet and triplet energy, transfer between lycopene and the BChl-as can occur by the Dexter, mechanism. The ring structure of the B850 BChl-as is optimal for light, energy transfer in that it samples all spatial absorption and emission, characteristics and places all oscillator strength into energetically low, lying, thermally accessible exciton states.
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<StructureSection load='1lgh' size='340' side='right'caption='[[1lgh]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lgh]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetospirillum_molischianum Magnetospirillum molischianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LGH FirstGlance]. <br>
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1LGH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Phaeospirillum_molischianum Phaeospirillum molischianum] with BCL, LYC, DET and HTO as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Sites: B80, B8A, B8B, E80, E8A, E8B, H80, H8A, H8B, K80, K8A and K8B. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LGH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=DET:UNDECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DET</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LYC:LYCOPENE'>LYC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgh OCA], [https://pdbe.org/1lgh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lgh RCSB], [https://www.ebi.ac.uk/pdbsum/1lgh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgh ProSAT]</span></td></tr>
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The crystal structure of the light-harvesting complex II (B800-850) from Rhodospirillum molischianum., Koepke J, Hu X, Muenke C, Schulten K, Michel H, Structure. 1996 May 15;4(5):581-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8736556 8736556]
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</table>
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[[Category: Phaeospirillum molischianum]]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/LHB1_MAGML LHB1_MAGML] Antenna complexes are light-harvesting systems, which transfer the excitation energy to the reaction centers.
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[[Category: Hu, X.]]
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== Evolutionary Conservation ==
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[[Category: Koepke, J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Michel, H.]]
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Check<jmol>
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[[Category: Schulten, K.]]
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<jmolCheckbox>
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[[Category: BCL]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/1lgh_consurf.spt"</scriptWhenChecked>
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[[Category: DET]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: HTO]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: LYC]]
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</jmolCheckbox>
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[[Category: bacteriochlorophyll]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lgh ConSurf].
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[[Category: dexter energy transfer]]
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<div style="clear:both"></div>
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[[Category: foerster exciton transfer mechanism]]
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__TOC__
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[[Category: light harvesting complex]]
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</StructureSection>
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[[Category: membrane protein]]
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[[Category: Large Structures]]
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[[Category: photosynthesis]]
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[[Category: Magnetospirillum molischianum]]
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[[Category: Hu X]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:42:29 2007''
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[[Category: Koepke J]]
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[[Category: Michel H]]
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[[Category: Schulten K]]

Current revision

CRYSTAL STRUCTURE OF THE LIGHT-HARVESTING COMPLEX II (B800-850) FROM RHODOSPIRILLUM MOLISCHIANUM

PDB ID 1lgh

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