5y9p
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5y9p is ON HOLD until Paper Publication Authors: Hang, T.R., Wu, M.H., Zhang, X.Z. Description: Staphylococcus aureus RNase HII [[Category: Unrelea...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Staphylococcus aureus RNase HII== | |
| + | <StructureSection load='5y9p' size='340' side='right'caption='[[5y9p]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5y9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y9P FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y9p OCA], [https://pdbe.org/5y9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y9p RCSB], [https://www.ebi.ac.uk/pdbsum/5y9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y9p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RNH2_STAA8 RNH2_STAA8] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.[HAMAP-Rule:MF_00052] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNase HII exists ubiquitously in organisms and functions as a monomer in prokaryotes. We determined the crystal structure of Staphylococcus aureus RNase HII (Sa-RNase HII), which displays a novel dimer conformation, with the active site of each monomer covered by the other one. Both small-angle X-ray scattering and gel-filtration analysis confirmed that Sa-RNase HII exists as a homodimer in solution. Enzymatic analysis revealed that the "self-inhibited" dimeric form is catalytically active. Furthermore, continuous-wave electron paramagnetic resonance experiments clarified that the Sa-RNase HII dimer undergoes a large conformational change upon substrate binding, but remains a dimer to catalyze the reaction. Our structural and biochemical studies identified a novel functional dimer of Sa-RNase HII with distinct regulation mechanism for its catalytic activity. | ||
| - | + | Structural insights into a novel functional dimer of Staphylococcus aureus RNase HII.,Hang T, Zhang X, Wu M, Wang C, Ling S, Xu L, Gong Q, Tian C, Zhang X, Zang J Biochem Biophys Res Commun. 2018 Jul 10. pii: S0006-291X(18)31521-3. doi:, 10.1016/j.bbrc.2018.07.026. PMID:30005877<ref>PMID:30005877</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5y9p" style="background-color:#fffaf0;"></div> |
| - | [[Category: Wu | + | |
| - | [[Category: | + | ==See Also== |
| + | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus]] | ||
| + | [[Category: Hang T]] | ||
| + | [[Category: Wu M]] | ||
| + | [[Category: Zhang X]] | ||
Current revision
Staphylococcus aureus RNase HII
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