1xsi

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[[Image:1xsi.gif|left|200px]]
 
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{{Structure
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==Structure of a Family 31 alpha glycosidase==
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|PDB= 1xsi |SIZE=350|CAPTION= <scene name='initialview01'>1xsi</scene>, resolution 2.20&Aring;
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<StructureSection load='1xsi' size='340' side='right'caption='[[1xsi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1xsi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSI FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= yicI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsi OCA], [https://pdbe.org/1xsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xsi RCSB], [https://www.ebi.ac.uk/pdbsum/1xsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xsi ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1xsj|1XSJ]], [[1xsk|1XSK]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsi OCA], [http://www.ebi.ac.uk/pdbsum/1xsi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xsi RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/XYLS_ECOLI XYLS_ECOLI] Can catalyze the transfer of alpha-xylosyl residue from alpha-xyloside to xylose, glucose, mannose, fructose, maltose, isomaltose, nigerose, kojibiose, sucrose and trehalose.<ref>PMID:15294295</ref> <ref>PMID:15501829</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xsi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xsi ConSurf].
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<div style="clear:both"></div>
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'''Structure of a Family 31 alpha glycosidase'''
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==See Also==
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*[[Xylosidase 3D structures|Xylosidase 3D structures]]
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== References ==
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==Overview==
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<references/>
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We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.
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__TOC__
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</StructureSection>
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==About this Structure==
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1XSI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSI OCA].
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==Reference==
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Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate., Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC, J Biol Chem. 2005 Jan 21;280(3):2105-15. Epub 2004 Oct 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15501829 15501829]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kim, Y W.]]
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[[Category: Kim YW]]
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[[Category: Lee, S S.]]
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[[Category: Lee SS]]
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[[Category: Lovering, A L.]]
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[[Category: Lovering AL]]
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[[Category: Strynadka, N C.]]
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[[Category: Strynadka NC]]
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[[Category: Withers, S G.]]
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[[Category: Withers SG]]
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[[Category: beta(8)alpha(8) barrel and beta sandwich domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:54:04 2008''
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Current revision

Structure of a Family 31 alpha glycosidase

PDB ID 1xsi

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