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1xsz

From Proteopedia

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[[Image:1xsz.gif|left|200px]]
 
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{{Structure
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==The structure of RalF==
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|PDB= 1xsz |SIZE=350|CAPTION= <scene name='initialview01'>1xsz</scene>, resolution 1.41&Aring;
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<StructureSection load='1xsz' size='340' side='right'caption='[[1xsz]], [[Resolution|resolution]] 1.41&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1xsz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSZ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.41&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsz OCA], [https://pdbe.org/1xsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xsz RCSB], [https://www.ebi.ac.uk/pdbsum/1xsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xsz ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsz OCA], [http://www.ebi.ac.uk/pdbsum/1xsz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xsz RCSB]</span>
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[https://www.uniprot.org/uniprot/Q8RT31_LEGPN Q8RT31_LEGPN]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''The structure of RalF'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xsz_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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The Legionella pneumophila protein RalF is secreted into host cytosol via the Dot/Icm type IV transporter where it acts to recruit ADP-ribosylation factor (Arf) to pathogen-containing phagosomes in the establishment of a replicative organelle. The presence in RalF of the Sec7 domain, present in all Arf guanine nucleotide exchange factors, has suggested that recruitment of Arf is an early step in pathogenesis. We have determined the crystal structure of RalF and of the isolated Sec7 domain and found that RalF is made up of two domains. The Sec7 domain is homologous to mammalian Sec7 domains. The C-terminal domain forms a cap over the active site in the Sec7 domain and contains a conserved folding motif, previously observed in adaptor subunits of vesicle coat complexes. The importance of the capping domain and of the glutamate in the "glutamic finger," conserved in all Sec7 domains, to RalF functions was examined using three different assays. These data highlight the functional importance of domains other than Sec7 in Arf guanine nucleotide exchange factors to biological activities and suggest novel mechanisms of regulation of those activities.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xsz ConSurf].
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1XSZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSZ OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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The structure of RalF, an ADP-ribosylation factor guanine nucleotide exchange factor from Legionella pneumophila, reveals the presence of a cap over the active site., Amor JC, Swails J, Zhu X, Roy CR, Nagai H, Ingmundson A, Cheng X, Kahn RA, J Biol Chem. 2005 Jan 14;280(2):1392-400. Epub 2004 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15520000 15520000]
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[[Category: Large Structures]]
[[Category: Legionella pneumophila]]
[[Category: Legionella pneumophila]]
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[[Category: Single protein]]
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[[Category: Amor JC]]
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[[Category: Amor, J C.]]
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[[Category: Cheng X]]
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[[Category: Cheng, X.]]
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[[Category: Ingmundson A]]
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[[Category: Ingmundson, A.]]
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[[Category: Kahn RA]]
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[[Category: Kahn, R A.]]
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[[Category: Nagai H]]
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[[Category: Nagai, H.]]
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[[Category: Roy CR]]
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[[Category: Roy, C R.]]
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[[Category: Swails J]]
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[[Category: Swails, J.]]
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[[Category: arf guanine nucleotide exchange factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:54:13 2008''
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Current revision

The structure of RalF

PDB ID 1xsz

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