5yer

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(New page: '''Unreleased structure''' The entry 5yer is ON HOLD Authors: Jo, I., Hong, S., Ahn, J., Ha, N.C. Description: Regulatory domain of HypT from Salmonella typhimurium (Bromide ion-bound)...)
Current revision (08:28, 22 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5yer is ON HOLD
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==Regulatory domain of HypT from Salmonella typhimurium (Bromide ion-bound)==
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<StructureSection load='5yer' size='340' side='right'caption='[[5yer]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5yer]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YER FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.301&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yer OCA], [https://pdbe.org/5yer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yer RCSB], [https://www.ebi.ac.uk/pdbsum/5yer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yer ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7CP75_SALTY Q7CP75_SALTY]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hypochlorous acid (HOCl) is generated in the immune system to kill microorganisms. In Escherichia coli, a hypochlorite-specific transcription regulator, HypT, has been characterized. HypT belongs to the LysR-type transcriptional regulator (LTTR) family that contains a DNA-binding domain (DBD) and a regulatory domain (RD). Here, we identified a hypT gene from Salmonella enterica serovar Typhimurium and determined crystal structures of the full-length HypT protein and the RD. The full-length structure reveals a type of tetrameric assembly in the LTTR family. Based on HOCl-bound and oxidation-mimicking structures, we identified a HOCl-driven methionine oxidation mechanism, in which the bound HOCl oxidizes a conserved methionine residue lining the putative ligand-binding site in the RD. Furthermore, we proposed a molecular model for the oxidized HypT, where methionine oxidation by HOCl results in a conformational change of the RD, inducing a counter rotation of the DBD dimers. Target genes that are regulated by HypT and their roles in Salmonella were also investigated. DNase I footprinting experiments revealed a DNA segment containing two pseudopalindromic motifs that are separated by approximately 100 bp, suggesting that only the oxidized structure makes a concomitant binding, forming a DNA loop. An understanding of the HypT-mediated mechanism would be helpful for controlling many pathogenic bacteria by counteracting bacterial HOCl defense mechanisms.
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Authors: Jo, I., Hong, S., Ahn, J., Ha, N.C.
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Structural basis for HOCl recognition and regulation mechanisms of HypT, a hypochlorite-specific transcriptional regulator.,Jo I, Kim D, No T, Hong S, Ahn J, Ryu S, Ha NC Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3740-3745. doi:, 10.1073/pnas.1811509116. Epub 2019 Feb 7. PMID:30733296<ref>PMID:30733296</ref>
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Description: Regulatory domain of HypT from Salmonella typhimurium (Bromide ion-bound)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Hong, S]]
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<div class="pdbe-citations 5yer" style="background-color:#fffaf0;"></div>
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[[Category: Jo, I]]
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== References ==
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[[Category: Ahn, J]]
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<references/>
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[[Category: Ha, N.C]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Ahn J]]
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[[Category: Ha NC]]
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[[Category: Hong S]]
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[[Category: Jo I]]

Current revision

Regulatory domain of HypT from Salmonella typhimurium (Bromide ion-bound)

PDB ID 5yer

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