6amg
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==cyt P460 of Nitrosomonas sp. AL212== | |
| + | <StructureSection load='6amg' size='340' side='right'caption='[[6amg]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6amg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_sp._AL212 Nitrosomonas sp. AL212]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AMG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6amg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6amg OCA], [https://pdbe.org/6amg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6amg RCSB], [https://www.ebi.ac.uk/pdbsum/6amg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6amg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F9ZFJ0_9PROT F9ZFJ0_9PROT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The enzymes hydroxylamine oxidoreductase and cytochrome (cyt) P460 contain related unconventional "heme P460" cofactors. These cofactors are unusual in their inclusion of nonstandard cross-links between amino acid side chains and the heme macrocycle. Mutagenesis studies performed on the Nitrosomonas europaea cyt P460 that remove its lysine-heme cross-link show that the cross-link is key to defining the spectroscopic properties and kinetic competence of the enzyme. However, exactly how this cross-link confers these features remains unclear. Here we report the 1.45 A crystal structure of cyt P460 from Nitrosomonas sp. AL212 and conclude that the cross-link does not lead to a change in hybridization of the heme carbon participating in the cross-link but rather enforces structural distortions to the macrocycle away from planarity. Time-dependent density functional theory coupled to experimental structural and spectroscopic analysis suggest that this geometric distortion is sufficient to define the spectroscopic properties of the heme P460 cofactor and provide clues toward establishing a relationship between heme P460 electronic structure and function. | ||
| - | + | The Eponymous Cofactors in Cytochrome P460s from Ammonia-Oxidizing Bacteria Are Iron Porphyrinoids Whose Macrocycles Are Dibasic.,Smith MA, Lancaster KM Biochemistry. 2017 Dec 6. doi: 10.1021/acs.biochem.7b00921. PMID:29211462<ref>PMID:29211462</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Lancaster | + | <div class="pdbe-citations 6amg" style="background-color:#fffaf0;"></div> |
| - | [[Category: Smith | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Nitrosomonas sp. AL212]] | ||
| + | [[Category: Lancaster K]] | ||
| + | [[Category: Smith M]] | ||
Current revision
cyt P460 of Nitrosomonas sp. AL212
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